Kelley G L, Youssef N N
Z Parasitenkd. 1977 Aug 25;53(1):23-9. doi: 10.1007/BF00383111.
Development of Eimeria vermiformis from sporozoite to mature first-generation schizonts in cultured bovine kidney cells, Madin-Darby bovine kidney cells, and primary cultures of whole mouse embryos is described. Intracellular sporozoites were seen at 5 min, and for as long as 120 h after inoculation. Sporozoites were observed penetrating cells, with uninucleate trophozoites and immature schizonts with 2--6 nuclei first appearing 24 h after inoculation. Schizonts with 6 or more nuclei, as well as mature schizonts containing first-generation merozoites, were first seen between 36 and 48 h after inoculation of all 3 cell types used. The first indication of merozoite formation was determined by the appearance of small protuberances of cytoplasm at the periphery of schizonts. Merozoites began development at the periphery of schizonts and were later observed radiating from a central body of cytoplasm, 14--20 merozoites being formed. Some mature schizonts retained a small spherical residual body after merozoite formation was completed. After the rupture of schizonts, intracellular merozoites, which contained anterior and posterior refractile granules, were seen at 48, 72 and 96 h postinoculation. Merozoites were not seen entering or leaving cells. No further development was observed.
本文描述了蠕形艾美球虫在培养的牛肾细胞、马迪-达比牛肾细胞和全鼠胚胎原代培养物中从子孢子发育为成熟第一代裂殖体的过程。接种后5分钟即可见到细胞内子孢子，且在接种后长达120小时内均可观察到。观察到子孢子侵入细胞，接种后24小时首次出现单核滋养体和含2 - 6个核的未成熟裂殖体。在接种所用的所有3种细胞类型后36至48小时之间，首次见到含6个或更多核的裂殖体以及含有第一代裂殖子的成熟裂殖体。裂殖子形成的最初迹象是裂殖体周边出现小的细胞质突起。裂殖子在裂殖体周边开始发育，随后观察到它们从细胞质中央体呈放射状排列，共形成14 - 20个裂殖子。一些成熟裂殖体在裂殖子形成完成后保留一个小的球形残余体。裂殖体破裂后，接种后48、72和96小时可见到细胞内含有前后折光颗粒的裂殖子。未观察到裂殖子进入或离开细胞。未观察到进一步的发育情况。
