LIRTS Viewer: A Web-Based Resource to View the Transcriptional Response of Lens Epithelial Cells to Injury.

PURPOSE

Residual lens epithelial cells (LECs) respond to injury after cataract surgery, leading to posterior capsular opacification (PCO). Transcriptomic profiling of lens capsule-associated cells (CACs) post-cataract surgery (PCS) revealed that LECs quickly alter their transcriptome, producing numerous pro-inflammatory cytokines within a few hours PCS. In contrast, the significant activation of TGFβ signaling and fibrotic extracellular matrix deposition related to PCO only begins 1 to 3 days later. However, the global changes in gene expression in CACs, following the establishment of robust TGFβ signaling, remain unknown.

METHODS

Lens fiber cells were removed from wild-type mice, and CACs were isolated at 0, 72, or 120 hours PCS to perform bulk RNA sequencing (RNA-seq) to obtain estimates of RNA abundance. These data were combined with existing RNA-seq datasets to create a web-based visualization resource to explore the expression dynamics of most protein coding genes in CACs.

RESULTS

At 72 hours PCS, CACs differentially express genes consistent with a surge in proliferation and changes in actin filament organization while also robustly expressing fibrotic marker genes by 120 hours PCS. We developed a data visualization resource, the Lens Injury Response Time Series (LIRTS) Viewer, which integrates all data to gather valuable insights from gene expression in CACs over the first 5 days PCS.

CONCLUSIONS

The LIRTS Viewer is useful for generating hypotheses related to PCO pathogenesis, as it reveals CAC gene expression dynamics, gene correlations, and biological pathways during the first 5 days following lens injury in an in vivo cataract surgery model.