猫急性局部缺血期间心肌中的脂质蓄积

Lipid accumulation in the myocardium during acute regional ischaemia in cats.

作者信息

Jodalen H, Stangeland L, Grong K, Vik-Mo H, Lekven J

出版信息

J Mol Cell Cardiol. 1985 Oct;17(10):973-80. doi: 10.1016/s0022-2828(85)80077-8.

Abstract

Accumulation of lipid material in the myocardium was studied in cat hearts with acute regional ischaemia of 3 h duration. The fractional volume of lipid droplets in cytosol was analysed by electron microscopy of myocardial biopsies using a quantitative stereologic technique. Ischaemic and normally perfused myocardium were identified by fluoresceine injection, and tissue blood flow measurements were performed with labelled microspheres. In normal myocardium only small amounts of lipid droplets were found. A marked accumulation of lipid droplets occurred in borderline tissue between the two types of myocardium, whereas lipid accumulation in ischaemic myocardium was less pronounced. The arterial concentration of nonesterified fatty acids was clearly increased during the 3 h coronary artery occlusion period. Increased triglyceride synthesis from arterial fatty acids, or redistribution of intracellular lipids, are suggested as possible explanations for lipid accumulation during acute myocardial ischaemia.

摘要

在持续3小时急性局部缺血的猫心脏中，研究了心肌中脂质物质的蓄积情况。采用定量体视学技术，通过心肌活检的电子显微镜分析细胞质中脂滴的分数体积。通过荧光素注射识别缺血心肌和正常灌注心肌，并用标记微球进行组织血流测量。在正常心肌中仅发现少量脂滴。在两种心肌之间的交界组织中出现了脂滴的明显蓄积，而缺血心肌中的脂质蓄积则不太明显。在冠状动脉闭塞的3小时期间，非酯化脂肪酸的动脉浓度明显升高。动脉脂肪酸合成甘油三酯增加或细胞内脂质重新分布，被认为是急性心肌缺血期间脂质蓄积的可能解释。