Muguerza-Guevara Kevin, Cortés-Acha Berta, García-García Marta, Figueiredo Rui, Soler-Ollé Agnès, Blanc Vanessa, Valmaseda-Castellón Eduard
Faculty of Medicine and Health Sciences, University of Barcelona, C/Feixa Llarga S/N, Campus de Bellvitge 2ª Planta, Despatx 2.9, Barcelona, 08907, Spain.
IDIBELL Research Institute, Barcelona, Spain.
BMC Oral Health. 2025 Jul 18;25(1):1201. doi: 10.1186/s12903-025-06580-4.
The microbiota associated with peri-implant diseases has been described, though information about biofilm formation and development on dental implants remains scarce.
To analyze and compare biofilm formation and distribution at 24 h, 48 h and 7 days on experimental abutments simulating dental implants in peri-implant healthy patients.
Experimental abutments with micro-threads and a modified rough surface were placed in healthy dental implants of 10 patients. Instructions were given not to clean the abutments for the duration of the study. Exclusion criteria included the use of antiseptics or antibiotics 30 days prior to recruitment or during the study period. After 24 h, 48 h and 7 days, the abutments were removed and stained using LIVE/DEAD stain, and two sides (buccal and palatal/lingual) and two areas (supragingival and subgingival) were assessed, with measurement of the mean biofilm covering area.
Twenty-nine experimental abutments placed in 10 patients were assessed. The total mean biomass coverage areas were 9.3%, 16.2% and 16.8% at 24 h, 48 h and 7 days, respectively, with significant differences being observed between 24 h and the subsequent timepoints (p < 0.05). Significantly greater supragingival biofilm coverage was observed at 7 days in comparison with the subgingival zone (21.85% versus 11.7%; p < 0.05).
Biofilm coverage on healthy dental implants increases progressively during the first 48 h and then stabilizes. The biofilm is mainly composed of live cells in the supragingival and subgingival areas. After 7 days, the supragingival areas show significantly greater biofilm coverage.
虽然已对与种植体周围疾病相关的微生物群进行了描述，但关于牙种植体上生物膜形成和发展的信息仍然匮乏。
分析并比较模拟牙种植体的实验基台在24小时、48小时和7天时，在种植体周围健康患者体内的生物膜形成和分布情况。
将带有微螺纹和改良粗糙表面的实验基台植入10例患者的健康牙种植体中。研究期间，嘱咐患者不要清洁基台。排除标准包括在入选前30天内或研究期间使用过防腐剂或抗生素。在24小时、48小时和7天后，取出基台，使用活菌/死菌染色剂进行染色，并评估两侧（颊侧和腭侧/舌侧）和两个区域（龈上和龈下），测量生物膜覆盖面积的平均值。
对10例患者植入的29个实验基台进行了评估。在24小时、48小时和7天时，生物膜总覆盖面积的平均值分别为9.3%、16.2%和16.8%，24小时与随后的时间点之间存在显著差异（p<0.05）。与龈下区域相比，7天时龈上生物膜覆盖面积显著更大（21.85%对11.7%；p<0.05）。
健康牙种植体上的生物膜覆盖面积在最初48小时内逐渐增加，然后趋于稳定。龈上和龈下区域的生物膜主要由活细胞组成。7天后，龈上区域的生物膜覆盖面积显著更大。
