Maleki Bahman, Shankaii Zainab, Salehi Abolfazl, Mohammadzadeh Tahereh
Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran; Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Radiation Sciences Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of Physiology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Ann Parasitol. 2025 Jul 15;71:63-69. doi: 10.17420/ap71.546.
Cystic echinococcosis (CE), a globally parasitic disease is primarily caused by the larval stage of Echinococcus granulosus. This zoonotic infection carries significant medical, veterinary, and economic implications. In human the disease occurs by the ingestion of parasite eggs and can create in any organ especially liver and lungs. Current therapeutic methods have some limitations. Therefore, effect of pulsed electric fields (PEF) as a non-invasive method was utilized on daughter cysts as an important part of hydatid cyst. Daughter cysts was extracted from sheep liver hydatid cyst, divided into three groups. The first group was exposed to PEF at intensities of 70V/cm, 150 V/cm and 1300V/cm. The second group was treated with albendazole (100 μg/ml). The third group, was exposed with different intensities of PEF (as above) following incubation of daughter cysts with albendazole (100 μg/ml). Structural changes in the daughter cysts were analyzed using a stereomicroscope. The laminated layer remained undamaged in all three groups. In the first group (PEF), some degree of opacity and detachment of germinal layer was observed especially in higher pulse intensity (1300 V/cm). Albendazole treatment (second group) induced significant opacity. In the combined PEF and albendazole (third group), although germinal layer detachment was occurred in addition to opacity but the level of detachment was not similar to those that observed in the first group. The destructive effect of PEF on the germinal layer of daughter cysts can be considered as a promising result in the treatment of CE.
囊型包虫病（CE）是一种全球性寄生虫病，主要由细粒棘球绦虫的幼虫阶段引起。这种人畜共患感染具有重大的医学、兽医和经济影响。在人类中，该疾病通过摄入寄生虫卵而发生，可在任何器官尤其是肝脏和肺部形成。目前的治疗方法存在一些局限性。因此，作为一种非侵入性方法的脉冲电场（PEF）对作为包虫囊肿重要组成部分的子囊的作用得到了研究。从绵羊肝脏包虫囊肿中提取子囊，分为三组。第一组分别暴露于强度为70V/cm、150V/cm和1300V/cm的PEF下。第二组用阿苯达唑（100μg/ml）处理。第三组在子囊与阿苯达唑（100μg/ml）孵育后，再暴露于不同强度的PEF（如上）下。使用体视显微镜分析子囊的结构变化。所有三组中的分层层均未受损。在第一组（PEF）中，尤其是在较高脉冲强度（1300V/cm）下，观察到生发层有一定程度的不透明和分离。阿苯达唑治疗（第二组）导致明显的不透明。在PEF和阿苯达唑联合治疗组（第三组）中，虽然除了不透明外还发生了生发层分离，但分离程度与第一组观察到的不同。PEF对子囊生发层的破坏作用可被视为囊型包虫病治疗中的一个有前景的结果。
