Hasegawa T, Ohta M, Kido N, Kato N, Miyamoto K, Koshiura R
Jpn J Pharmacol. 1985 Aug;38(4):355-60. doi: 10.1254/jjp.38.355.
The antitumor activity of the polysaccharide fraction (OPS) obtained by the acid hydrolysis of Klebsiella O3 lipopolysaccharide (KO3 LPS) isolated from the culture supernatant of the decapsulated mutant strain LEN-1 (03: K1-) against both allogeneic tumor and syngeneic tumor systems in mice was compared with that of KO3 LPS. OPS prolonged the life span of MM2-bearing C3H/He mice by intraperitoneal (i.p.) pre- and post-treatment at the doses of 100 and 1000 mg/kg. However, large amounts of OPS were needed to show the antitumor activity as compared with KO3 LPS. OPS showed no growth inhibitory activity against Meth-A sarcoma in BALB/c mice by i.p., intravenous (i.v.) or intratumoral (i.t.) administration. When 1000 mg/kg of OPS was i.p. administered once a day for 10 days, OPS significantly inhibited the tumor growth of Sarcoma-180 solid type tumor. On the other hand, KO3 LPS significantly suppressed the growth of Meth-A tumor by i.t. administration at the doses of 0.3 and 1.0 mg/kg and showed complete regression in 8 and 9 out of 10 mice, respectively. In MM2 tumor, KO3 LPS also showed complete regression in all mice post-treated by i.p. administration at the dose of 1.0 mg/kg. These results suggest that OPS has antitumor activity on the tumors used in this study, but the activity was less than that of KO3 LPS.
将从脱荚膜突变株LEN-1（O3：K1-）培养上清液中分离得到的肺炎克雷伯菌O3脂多糖（KO3 LPS）经酸水解获得的多糖组分（OPS）对小鼠同种异体肿瘤和同基因肿瘤系统的抗肿瘤活性与KO3 LPS进行了比较。OPS通过腹腔内（i.p.）预处理和后处理，以100和1000 mg/kg的剂量延长了荷MM2的C3H/He小鼠的寿命。然而，与KO3 LPS相比，需要大量的OPS才能显示出抗肿瘤活性。OPS通过腹腔内、静脉内（i.v.）或瘤内（i.t.）给药对BALB/c小鼠的Meth-A肉瘤没有生长抑制活性。当每天腹腔注射1000 mg/kg的OPS，连续注射10天时，OPS显著抑制了肉瘤-180实体型肿瘤的生长。另一方面，KO3 LPS通过瘤内给药，剂量为0.3和1.0 mg/kg时，显著抑制了Meth-A肿瘤的生长，分别在10只小鼠中有8只和9只出现完全消退。在MM2肿瘤中，KO3 LPS以1.0 mg/kg的剂量腹腔内给药后处理所有小鼠，也出现了完全消退。这些结果表明，OPS对本研究中使用的肿瘤具有抗肿瘤活性，但活性低于KO3 LPS。
