Miyamoto K, Koshiura R, Hasegawa T, Kato N
Jpn J Pharmacol. 1984 Sep;36(1):51-7. doi: 10.1254/jjp.36.51.
The antitumor activity of Klebsiella 03 lipopolysaccharide (KO3 LPS) isolated from the culture supernatant against S180 sarcoma, Ehrlich carcinoma, MM2 mammary carcinoma and Meth A fibrosarcoma in mice was investigated. KO3 LPS significantly prolonged the lifespan of S180-bearing ddY mice and MM2-bearing C3H/He mice by intraperitoneal pre- or postmedication at doses ranging from 0.1 to 1.0 mg/kg. The LPS also inhibited the growth of subcutaneously inoculated Ehrlich carcinoma in ddY mice and Meth A sarcoma in BALB/c mice by intraperitoneal, intravenous or intratumoral administration. The intratumoral injection of KO3 LPS was most effective and results by the intravenous and the intraperitoneal administrations followed in effectiveness, but the administration through the subcutaneous route was hardly effective. Thus, KO3 LPS was shown to have antitumor activity on both allogeneic tumors and syngeneic tumors. It was also indicated in this study that the lifeprolonging effect of KO3 LPS on S180 ascites type tumor-bearing mice was significantly minimized by pretreatment of cyclophosphamide and that the LPS did not influence the cell viability of HeLa cells, Ehrlich cells and MM2 cells in vitro. These results suggest that the antitumor activity of KO3 LPS is provided by host-mediated actions.
研究了从培养上清液中分离出的肺炎克雷伯菌03脂多糖（KO3 LPS）对小鼠S180肉瘤、艾氏癌、MM2乳腺癌和Meth A纤维肉瘤的抗肿瘤活性。KO3 LPS通过腹腔内给药，在0.1至1.0 mg/kg的剂量下，对荷S180的ddY小鼠和荷MM2的C3H/He小鼠进行预给药或后给药，可显著延长其生存期。该脂多糖通过腹腔内、静脉内或瘤内给药，还可抑制ddY小鼠皮下接种的艾氏癌和BALB/c小鼠的Meth A肉瘤的生长。瘤内注射KO3 LPS最有效，静脉内和腹腔内给药的效果次之，但皮下给药几乎无效。因此，KO3 LPS对同种异体肿瘤和同基因肿瘤均具有抗肿瘤活性。本研究还表明，环磷酰胺预处理可显著降低KO3 LPS对荷S180腹水型肿瘤小鼠的生存期延长作用，且该脂多糖在体外不影响HeLa细胞、艾氏细胞和MM2细胞的细胞活力。这些结果表明，KO3 LPS的抗肿瘤活性是由宿主介导的作用产生的。
