Tianli Wang, Li Shaojing, Zhang Rong
School of Health, Shaanxi Institute of Fashion Engineering, Xi'an, Shaanxi, 712046, China.
Jinzhou Medical University Graduate Training Base, Jinzhou City, Liaoning Province, China.
J Ovarian Res. 2025 Jul 19;18(1):157. doi: 10.1186/s13048-025-01752-w.
To investigate the expression of LAMA 5 (laminin subunit α5) in high-grade serous ovarian cancer and its relationship with the prognosis of ovarian cancer patients.
The expression of LAMA5 in high-grade serous ovarian cancer was explored through the ovarian cancer sample information in multiple databases and real-time fluorescent quantitative PCR (Real Time Quantitative PCR, RT-qPCR). Effect of LAMA5 on the proliferation and migration of ovarian cancer cells. The possible mechanism of LAMA 5 affecting the malignant process of ovarian cancer was searched by GSEA analysis.
Based on bioinformatics analysis and RT-qPCR verification in multiple databases, it was found that LAMA5 was highly expressed in serous ovarian cancer, which was related to clinical stage and indicated poor prognosis. High expression of LAMA 5 can promote the proliferation and migration of ovarian cancer cells. Based on the GSEA analysis, it is suggested that the process of LAMA5 promoting the occurrence and development of ovarian cancer may be related to the mitotic pathway of the spindle (Mitotic Spindle) and the Hedgehog (Hh) signaling pathway.
LAMA 5 may be used as a clinical marker for evaluating the prognosis of serous ovarian cancer and a new therapeutic target.
探讨层粘连蛋白α5（LAMA 5）在高级别浆液性卵巢癌中的表达及其与卵巢癌患者预后的关系。
通过多个数据库中的卵巢癌样本信息及实时荧光定量PCR（RT-qPCR）探究LAMA5在高级别浆液性卵巢癌中的表达。观察LAMA5对卵巢癌细胞增殖和迁移的影响。通过基因集富集分析（GSEA）寻找LAMA 5影响卵巢癌恶性进程的可能机制。
基于多个数据库的生物信息学分析及RT-qPCR验证，发现LAMA5在浆液性卵巢癌中高表达，与临床分期相关且提示预后不良。LAMA 5高表达可促进卵巢癌细胞的增殖和迁移。基于GSEA分析，提示LAMA5促进卵巢癌发生发展的过程可能与纺锤体有丝分裂途径（Mitotic Spindle）和刺猬信号通路（Hh）有关。
LAMA 5可能作为评估浆液性卵巢癌预后的临床标志物及新的治疗靶点。
