协同莫西沙星检测：具有定向抗体固定化的AgNPs/GO电化学免疫传感器。

Synergistic moxifloxacin detection: AgNPs/GO electrochemical immunosensor with oriented antibody immobilization.

作者信息

Chen Yumei, Wang Zemin, Zhang Gaiping, Zhou Yiting, Zhou Jingming, Liang Chao, Wu Sixuan, Liu Hongliang, Ma Zhanqun, Zhu Xifang, Liu Enping, Wang Aiping

机构信息

Longhu Laboratory of Advanced Immunology, Zhengzhou, Henan, China; School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China; Henan Key Laboratory of Immunobiology, Zhengzhou, Henan, China.

出版信息

Food Chem. 2025 Nov 15;492(Pt 3):145424. doi: 10.1016/j.foodchem.2025.145424. Epub 2025 Jul 3.

DOI:10.1016/j.foodchem.2025.145424

PMID:40684568

Abstract

Moxifloxacin (MXF), a broad-spectrum fluoroquinolone antibiotic, is widely used in human and veterinary medicine. However, its overuse leads to environmental residues and antimicrobial resistance, posing significant health risks. Herein, a label-free electrochemical immunosensor was developed for ultrasensitive MXF detection using silver nanoparticle-graphene oxide nanocomposites (AgNPs/GO). The synergistic effects of GO (high conductivity) and AgNPs (redox activity) enhanced electron transfer efficiency. Critically, Staphylococcal protein A (SPA) enabled oriented Fc-directed antibody immobilization, significantly enhancing antigen-binding efficiency and sensitivity compared to random adsorption. Material characterization (UV-Vis, XRD, SEM, TEM) confirmed the nanostructure. The sensor exhibited a wide linear range (0.50-200 ng/mL), ultralow detection limit (0.18 ng/mL, S/N = 3), and high specificity against structural analogs. Practical validation in spiked pork and milk samples achieved recoveries of 95-104 % with long-term stability (>82.5 % signal retention over 28 days). This work provides a reliable platform for monitoring antibiotic residues in food safety systems.

摘要

莫西沙星（MXF）是一种广谱氟喹诺酮类抗生素，广泛应用于人类医学和兽医学。然而，其过度使用导致环境残留和抗菌耐药性，带来重大健康风险。在此，开发了一种无标记电化学免疫传感器，用于使用银纳米颗粒-氧化石墨烯纳米复合材料（AgNPs/GO）超灵敏检测MXF。GO（高导电性）和AgNPs（氧化还原活性）的协同效应提高了电子转移效率。至关重要的是，葡萄球菌蛋白A（SPA）实现了Fc导向抗体的定向固定，与随机吸附相比，显著提高了抗原结合效率和灵敏度。材料表征（紫外-可见光谱、X射线衍射、扫描电子显微镜、透射电子显微镜）证实了纳米结构。该传感器表现出宽线性范围（0.50-200 ng/mL）、超低检测限（0.18 ng/mL，S/N = 3）以及对结构类似物的高特异性。在加标猪肉和牛奶样品中的实际验证实现了95-104%的回收率，且具有长期稳定性（28天内信号保留率>82.5%）。这项工作为食品安全系统中监测抗生素残留提供了一个可靠的平台。