Bouby N, Bankir L, Trinh-Trang-Tan M M, Minuth W W, Kriz W
Kidney Int. 1985 Sep;28(3):456-66. doi: 10.1038/ki.1985.152.
A morphometric study was undertaken to quantitate the morphologic changes induced by ADH availability in the rat kidney. Homozygous Brattleboro rats with hereditary diabetes insipidus (DI) (no ADH) were compared to heterozygous Brattleboro control rats (HZ) and to DI rats after 5 to 6 weeks of continuous ADH infusion by implantable Alzet osmotic minipumps (TDI). ADH resulted in a 37% increase in mass of kidney per unit body wt. All kidney zones and all nephron segments were not increased uniformly. The inner stripe was enlarged more than other renal zones. It represented 15.5 +/- 0.7% of the total kidney height along the cortico-papillary axis in DI and 22.2 +/- 1.5% in TDI (P less than 0.025). The volume of the inner stripe in DI and TDI amounted to 10.9 +/- 0.9 and 18.0 +/- 1.0% of the total kidney volume, respectively (P less than 0.001). Selective increases in tubular diameter and cell height, due mostly to an hypertrophy of pre-existing cells, were observed in the earliest part of the thick ascending limbs (TAL) in the inner stripe, resulting in a twofold increase in epithelial volume per unit tubular length (P less than 0.001). Volume density of mitochondria and surface density of basolateral membranes were unchanged but, due to the increase in cell volume and inner stripe thickness, the amount of mitochondria and the surface area of basolateral membrane in the TAL were more than tripled in the inner stripe of treated rats. These changes provide a much greater salt transport capacity in the TAL of treated rats. They probably represent an adaptation of the early TAL to an enhanced sodium chloride transport in response to a direct ADH stimulation and/or to an increased salt delivery to this segment in the concentrating kidney.
进行了一项形态计量学研究，以量化抗利尿激素（ADH）可用性对大鼠肾脏诱导的形态学变化。将患有遗传性尿崩症（DI）（无ADH）的纯合子布拉特洛维大鼠与杂合子布拉特洛维对照大鼠（HZ）以及通过可植入的Alzet渗透微型泵连续输注ADH 5至6周后的DI大鼠（TDI）进行比较。ADH导致单位体重的肾脏质量增加37%。所有肾区和所有肾单位节段并非均匀增加。内带比其他肾区增大得更多。在皮质-乳头轴上，它在DI大鼠中占肾脏总高度的15.5±0.7%，在TDI大鼠中占22.2±1.5%（P<0.025）。DI和TDI大鼠内带的体积分别占肾脏总体积的10.9±0.9%和18.0±1.0%（P<0.001）。在内带的厚升支（TAL）最早部分观察到管腔直径和细胞高度选择性增加，这主要是由于原有细胞肥大所致，导致单位管长的上皮体积增加了两倍（P<0.001）。线粒体的体积密度和基底外侧膜的表面密度没有变化，但由于细胞体积和内带厚度增加，治疗大鼠内带TAL中的线粒体数量和基底外侧膜的表面积增加了两倍多。这些变化使治疗大鼠的TAL具有更大的盐转运能力。它们可能代表早期TAL对直接ADH刺激和/或浓缩肾中该节段盐输送增加的反应，以增强氯化钠转运的一种适应性变化。
