Saidu Sarah, Obeng Forson Akua, Appiah-Kwarteng Cornelia, Oduro-Mensah Daniel, Olu-Taiwo Michael, Ntiwaa Quarchie Marjorie, Osei Mary-Magdalene, Kwao Paul, Obeng-Nkrumah Noah
Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana.
School of Veterinary Medicine, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana.
Microb Drug Resist. 2025 Aug;31(8):231-240. doi: 10.1177/10766294251360947. Epub 2025 Jul 21.
We investigated fecal colonization with third-generation cephalosporin-resistant (3GC-r) Enterobacterales and carbapenem-resistant Enterobacterales among food animals and their handlers in Ghana. A total of 252 fecal samples were collected from 211 animals and 41 human handlers across 20 farms between May and August 2023. Enterobacterales were isolated using standard methods and identified using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry fingerprints and 16SrRNA sequencing. Antibiotic susceptibility testing was done using standard methods. Extended spectrum beta-lactamase (ESBL) and carbapenemase genes were identified by PCR and sequencing. A total of 264 Enterobacterales were isolated, comprising 44 from human handlers and 220 from food animals. Among human isolates, 31 (70.5%) were 3GC-r, with 9 (20.5%) expressing the ESBL phenotype and 2 (4.5%) producing carbapenemases. The most common ESBL genes detected were ( = 5/9) and ( = 2/9), while carbapenemase-producing isolates harbored ( = 1/2) or ( = 1/2). In food animals, 94 (42.7%) of Enterobacterales isolates were 3GC-r, with 11 (5.0%) carrying ESBL genes, predominantly ( = 5/11). One isolate exhibited carbapenemase production () with an ESBL gene (). Meropenem was the most effective antibiotic agent against the study isolates (≤5% resistance). Concordance of isolate/resistance gene combinations was observed at three farms where at least one human handler and at least one farm animal carried the same 3GC-r with the same resistance genes, including , , and The findings indicate a potential for zoonotic transmission of resistance genes between food animals and their human handlers.
我们调查了加纳食用动物及其饲养者中对第三代头孢菌素耐药（3GC-r）的肠杆菌科细菌和耐碳青霉烯类肠杆菌科细菌的粪便定植情况。2023年5月至8月期间，从20个农场的211只动物和41名人类饲养者中总共采集了252份粪便样本。使用标准方法分离肠杆菌科细菌，并通过基质辅助激光解吸/电离飞行时间质谱指纹图谱和16SrRNA测序进行鉴定。采用标准方法进行抗生素敏感性试验。通过PCR和测序鉴定超广谱β-内酰胺酶（ESBL）和碳青霉烯酶基因。总共分离出264株肠杆菌科细菌，其中44株来自人类饲养者，220株来自食用动物。在人类分离株中，31株（70.5%）对3GC耐药，其中9株（20.5%）表现出ESBL表型，2株（4.5%）产生碳青霉烯酶。检测到的最常见ESBL基因是（=5/9）和（=2/9），而产碳青霉烯酶的分离株携带（=1/2）或（=1/2）。在食用动物中，94株（42.7%）肠杆菌科细菌分离株对3GC耐药，11株（5.0%）携带ESBL基因，主要是（=5/11）。一株分离株（）产生碳青霉烯酶，并带有一个ESBL基因（）。美罗培南是对研究分离株最有效的抗生素（耐药率≤5%）。在三个农场观察到分离株/耐药基因组合的一致性，在这些农场中，至少有一名人类饲养者和至少一头农场动物携带相同的3GC-r和相同的耐药基因，包括、和。研究结果表明，耐药基因在食用动物及其人类饲养者之间存在人畜共患传播的可能性。
