阿尔及利亚家畜中耐多药大肠杆菌的粪便携带情况：新德里金属β-内酰胺酶（NDM）和OXA-181型碳青霉烯酶的出现

Fecal carriage of resistant Escherichia coli in livestock in Algeria: emergence of NDM and OXA-181.

作者信息

Kirat Hassina, Rahab Hamza, Chekroud Zohra, Abbassi Mohamed Salah, Basher Nosiba S, Ibrahim Nasir Adam, Touati Abdelaziz

机构信息

Laboratoire de recherche des Interactions, Biodiversité, Écosystèmes et Biotechnologie. Faculté des Sciences. Département des sciences de la nature et de la vie, Université 20 Aout 1955, Skikda, Algeria.

Équipe santé animale. Division biotechnologie et santé, Centre de recherche en biotechnologie Constantine, Constantine, Algeria.

出版信息

BMC Microbiol. 2025 Aug 1;25(1):473. doi: 10.1186/s12866-025-04174-2.

DOI:10.1186/s12866-025-04174-2

PMID:40751129

Abstract

INTRODUCTION

The spread of third-generation cephalosporin (3GC)-resistant Escherichia coli in food-producing animals poses a significant threat to public health, with limited data from cattle and sheep in Algeria. This study investigated the prevalence of 3GC-resistant E. coli in cattle and sheep in Guelma, northeast Algeria.

METHODOLOGY

Two hundred eighty-five fecal samples were collected from cattle (n=145) and sheep (n=140) on 28 farms. Samples were screened for 3GC-resistant E. coli. Antibiotic susceptibility was tested, and ESBL and carbapenemase production were evaluated using double disc and EDTA tests. PCR identified resistance and integron genes.

RESULTS

Twenty-seven cefotaxime-resistant E. coli isolates were detected in 17% of bovine and 1% of ovine samples, spanning 43% of the farms. Multidrug resistance was observed in 85% of isolates, with high resistance to β-lactams, tetracyclines, fluoroquinolones, and trimethoprim-sulfamethoxazole. The following beta-lactamase genes were detected: bla (74%), bla (44%), bla (37%), and bla (4%) were identified. Class 1 integrons were also detected in ten isolates.

CONCLUSIONS

These findings emphasize the presence of ESBL-, AmpC-, and carbapenemase-producing E. coli among Algerian livestock, highlighting the need for comprehensive monitoring and control to manage the spread of these resistant bacteria.

摘要

引言

产食动物中对第三代头孢菌素（3GC）耐药的大肠杆菌传播对公众健康构成重大威胁，而阿尔及利亚牛和羊的相关数据有限。本研究调查了阿尔及利亚东北部盖勒马地区牛和羊中对3GC耐药的大肠杆菌的流行情况。

方法

从28个农场的牛（n = 145）和羊（n = 140）中采集了285份粪便样本。对样本进行了对3GC耐药的大肠杆菌筛查。测试了抗生素敏感性，并使用双纸片和EDTA试验评估了超广谱β-内酰胺酶（ESBL）和碳青霉烯酶的产生情况。通过聚合酶链反应（PCR）鉴定耐药和整合子基因。

结果

在17%的牛样本和1%的羊样本中检测到27株对头孢噻肟耐药的大肠杆菌分离株，涉及43%的农场。85%的分离株表现出多重耐药，对β-内酰胺类、四环素类、氟喹诺酮类和甲氧苄啶-磺胺甲恶唑具有高度耐药性。检测到以下β-内酰胺酶基因：bla（74%）、bla（44%）、bla（37%）和bla（4%）。在10株分离株中也检测到1类整合子。