通过商业酶和新型NYO衍生酶的创新协同作用生产大豆苷元作为替代雌激素。
Production of soy aglycones as an alternative estrogen via innovative synergy of commercial and novel NYO-derived enzymes.
作者信息
Seong Hyeon-Jun, Bharti Deepti, Kim Seong Gyung, Yang Kwang-Yeol, Nam Seung-Hee
机构信息
Department of Integrative Food, Bioscience, and Biotechnology, Chonnam National University, Gwangju 61186, South Korea.
Graduate School of International Agricultural Technology, Seoul National University, Gangwon-do 25354, South Korea.
出版信息
Food Chem X. 2025 Jul 4;29:102732. doi: 10.1016/j.fochx.2025.102732. eCollection 2025 Jul.
Soy isoflavones help mitigate cancer and estrogen-related diseases. This study aimed to improve raw soybean functionality by optimizing enzymatic biotransformation to produce soy aglycones, primarily daidzein and genistein, which resemble endogenous estrogen. Initial screening optimized commercial and fermented food-derived enzymes to boost isoflavone and aglycone content, respectively. The sequential application of Pluszyme 2000P© and NYO maximized isoflavone extraction (4650 μg/g) and enhanced aglycone content 3.6 times compared to the non-treated control. Soy aglycone showed improved physicochemical properties and a compact, uniform structure, as revealed by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Electronic nose profiling showed reduced off-flavors (acidic, grassy) and enhanced desirable volatiles (sweet, caramelized). Biofunctionally, the soy aglycone elicited estrogen-like effects, promoting MCF-7 cell proliferation by 36 % without triggering cancerous growth. Estrogen receptor levels reached 14.87 pg/mg, approximately 6.5 times higher than untreated soy, supporting its potential as a safe phytoestrogen source.
大豆异黄酮有助于减轻癌症和雌激素相关疾病。本研究旨在通过优化酶促生物转化来提高生大豆的功能，以生产大豆苷元，主要是大豆苷和染料木黄酮，它们类似于内源性雌激素。初步筛选优化了商业和发酵食品来源的酶，分别提高异黄酮和苷元含量。与未处理的对照相比，依次应用Pluszyme 2000P©和NYO可使异黄酮提取量最大化（4650μg/g），并使苷元含量提高3.6倍。傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）显示，大豆苷元具有改善的物理化学性质和致密、均匀的结构。电子鼻分析表明，异味（酸性、青草味）减少， desirable挥发物（甜味、焦糖味）增加。在生物功能方面，大豆苷元产生类似雌激素的作用，促进MCF-7细胞增殖36%，而不引发癌细胞生长。雌激素受体水平达到14.87pg/mg，约为未处理大豆的6.5倍，支持其作为安全植物雌激素来源的潜力。
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