Liu Yuxuan, Jiang Kang, Li Xiaoli, Lu Linfang, Sun Menghan, Li Na, Madushika Lakshani, Yuan Jun, Ling Sumei, Wang Shihua
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Food Chem X. 2025 Jul 9;29:102771. doi: 10.1016/j.fochx.2025.102771. eCollection 2025 Jul.
Flumequine (Flu) is a fluoroquinolone veterinary antibiotic, which is easy to accumulate in animals, and Flu residues may cause a latent risk to human physiological health. In this study, a high-affinity monoclonal antibody (2.09 × 10 M) to specifically recognize Flu (anti-Flu mAb) was prepared, and then the lateral flow immunochromatographic strips (LFIS) with excellent sensitivity and specificity were developed by combining mAbs with nanoparticles. The linear detection ranges of LFIS based on gold particles (AuNP-LFIS) and gold nanoflowers (AuNF-LFIS) were 1.95-250 ng/mL and 0.39-100 ng/mL, respectively, and two types of LFIS showed high sensitivity and accuracy in actual sample detection. It is noteworthy that the AuNF-LFIS exhibited a higher sensitivity compared to that of the AuNP-LFIS. The LFIS developed in this study were considered to have great application potential in monitoring food safety because of its superiorities including simple operation, high sensitivity, prompted speed and good specificity.
氟甲喹（Flu）是一种氟喹诺酮类兽用抗生素，容易在动物体内蓄积，其残留可能对人体生理健康造成潜在风险。本研究制备了一种高亲和力的特异性识别氟甲喹的单克隆抗体（2.09×10⁻⁹M）（抗氟甲喹单克隆抗体），然后将该单克隆抗体与纳米颗粒结合，研制出具有优异灵敏度和特异性的侧向流动免疫层析试纸条（LFIS）。基于金颗粒的LFIS（AuNP-LFIS）和金纳米花的LFIS（AuNF-LFIS）的线性检测范围分别为1.95 - 250 ng/mL和0.39 - 100 ng/mL，两种类型的LFIS在实际样品检测中均表现出高灵敏度和准确性。值得注意的是，AuNF-LFIS比AuNP-LFIS表现出更高的灵敏度。本研究研制的LFIS因其操作简单、灵敏度高、检测速度快和特异性好等优势，被认为在食品安全监测方面具有巨大的应用潜力。
