da Silva Thamyres Freire, Ximenes Mesquita Jacilane, Luz Erika Patricia Chagas Gomes, Andrade Alexandre Lopes, Kobs Henry, Teixeira Edson Holanda, de Souza Filho Antônio Gomes, de Faria Andreia Fonseca, Mattos Adriano Lincoln Albuquerque, Andrade Fábia Karine, Silveira Vieira Rodrigo
Adsorption Separations Research Group, Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-760, Brazil.
Integrated Biomolecules Laboratory, Department of Pathology and Forensic Medicine, Federal University of Ceará, Fortaleza- Ceará 60430350, Brazil.
ACS Omega. 2025 Jun 30;10(27):28969-28981. doi: 10.1021/acsomega.5c00609. eCollection 2025 Jul 15.
Patients dealing with chronic wounds frequently experience persistent pain and a heightened risk of infection due to microbial contamination. To improve the quality of life for these patients while reducing treatment duration and costs, advanced wound dressings are being developed. This study aimed to develop a wound dressing from natural bacterial-derived cellulose fibers (BC) and calcium alginate (A), which were functionalized with silver-containing graphene oxide nanoparticles (GOAg). Carbon-based nanomaterials, such as GOAg, are recognized for their antimicrobial properties, noncytotoxic nature, and their strong ability to absorb exudate, which is crucial for controlling infections in dermal lesions. In vitro testing revealed that the BC-A-GOAg dressing possessed optimized characteristics, including a uniform distribution of GOAg nanoparticles within the polymeric blend of BC and A. When in direct contact with bacterial cells in suspension, the BC-A-GOAg dressing exhibited 74% antimicrobial activity against and 59% against . Additionally, the antimicrobial BC-A-GOAg demonstrated no significant cytotoxicity to mouse fibroblast cells (L-929), maintaining 90.8% ± 5.2% cell viability after 48 h of exposure. Furthermore, the in vitro assessments showed that the BC-A-GOAg dressing could inhibit the activity of the myeloperoxidase enzyme, highlighting its effectiveness in reducing inflammation.
患有慢性伤口的患者经常会经历持续的疼痛，并且由于微生物污染而面临更高的感染风险。为了提高这些患者的生活质量，同时缩短治疗时间并降低成本，人们正在研发先进的伤口敷料。本研究旨在利用天然细菌衍生的纤维素纤维（BC）和海藻酸钙（A）开发一种伤口敷料，并用含银的氧化石墨烯纳米颗粒（GOAg）对其进行功能化处理。碳基纳米材料，如GOAg，因其抗菌性能、无细胞毒性以及强大的吸收渗出液的能力而受到认可，这对于控制皮肤损伤中的感染至关重要。体外测试表明，BC-A-GOAg敷料具有优化的特性，包括GOAg纳米颗粒在BC和A的聚合物共混物中均匀分布。当与悬浮液中的细菌细胞直接接触时，BC-A-GOAg敷料对[具体细菌1]表现出74%的抗菌活性，对[具体细菌2]表现出59%的抗菌活性。此外，抗菌的BC-A-GOAg对小鼠成纤维细胞（L-929）没有明显的细胞毒性，在暴露48小时后细胞活力维持在90.8%±5.2%。此外，体外评估表明，BC-A-GOAg敷料可以抑制髓过氧化物酶的活性，突出了其在减轻炎症方面的有效性。
