Vasconcelos Niédja Fittipaldi, Chevallier Pascale, Mantovani Diego, Rosa Morsyleide de Freitas, Barros Fernando José Soares, Andrade Fábia Karine, Vieira Rodrigo Silveira
Centro de Tecnologias Estratégicas do Nordeste (CETENE), Laboratório de Materiais Nanoestruturados (LMNano), Cidade Universitária, Avenida Professor Luiz Freire 01, Recife 50740-540, PE, Brazil.
Laboratory for Biomaterials & Bioengineering (LBB), Department of Min-Met-Materials Engineering & CHU de Quebec Research Center, Laval University, Quebec, QC G1V 0A6, Canada.
Pharmaceutics. 2024 Aug 18;16(8):1085. doi: 10.3390/pharmaceutics16081085.
This research consolidates our group's advances in developing a therapeutic dressing with innovative enzymatic debridement, focusing on the physicochemical and in vitro biological properties of papain immobilized in wet oxidized bacterial cellulose (OxBC-Papain) dressing. OxBC membranes were produced with oxidized with NaIO, and papain was immobilized on them. They were characterized in terms of enzyme stability (over 100 days), absorption capacity, water vapor transmission (WVT), hemocompatibility, cytotoxicity, and cell adhesion. The OxBC-Papain membrane showed 68.5% proteolytic activity after 100 days, demonstrating the benefit of using the OxBC wet membrane for papain stability. It had a WVT rate of 678 g/m·24 h and cell viability of 99% and 86% for L929 and HaCat cells, respectively. The membranes exhibited non-hemolytic behavior and maintained 26% clotting capacity after 1 h. The wet OxBC-Papain membrane shows significant potential as a natural biomolecule-based therapeutic dressing for wound care, offering efficient debridement, moisture maintenance, exudate absorption, gas exchange, and hemostasis without cytotoxic effects or cell adhesion to the dressing. Further research, especially using in vivo models, is needed to assess its efficacy in inducing epithelialization. This study advances stomatherapy knowledge, providing a cost-effective solution for enzymatic debridement in healthcare.
本研究巩固了我们团队在开发具有创新酶促清创功能的治疗性敷料方面的进展，重点关注固定在湿氧化细菌纤维素（OxBC-木瓜蛋白酶）敷料中的木瓜蛋白酶的物理化学和体外生物学特性。用NaIO氧化制备OxBC膜，并将木瓜蛋白酶固定在其上。对其进行了酶稳定性（超过100天）、吸收能力、水蒸气透过率（WVT）、血液相容性、细胞毒性和细胞黏附等方面的表征。OxBC-木瓜蛋白酶膜在100天后显示出68.5%的蛋白水解活性，证明了使用OxBC湿膜对木瓜蛋白酶稳定性的益处。它的WVT率为678 g/m·24 h，L929细胞和HaCat细胞的细胞活力分别为99%和86%。这些膜表现出非溶血行为，1小时后凝血能力维持在26%。湿的OxBC-木瓜蛋白酶膜作为一种基于天然生物分子的伤口护理治疗性敷料具有巨大潜力，可提供高效清创、保持水分、吸收渗出液、气体交换和止血功能，且无细胞毒性作用或细胞黏附于敷料。需要进一步研究，尤其是使用体内模型，以评估其诱导上皮化的功效。本研究推进了伤口治疗学知识，为医疗保健中的酶促清创提供了一种经济有效的解决方案。
