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瑞士食用的野猪()肌肉中的物种及…… (原文此处不完整)

spp. and in muscles from wild boars () consumed in Switzerland.

作者信息

Gastón Moré, Carlotta Filippini, Oehm Andreas W, Maja Ruetten, Andrew Hemphill, Frey Caroline F, Walter Basso

机构信息

Institute of Parasitology, University of Bern, Länggassstrasse 122, 3012, Bern, Switzerland.

National Scientific and Technical Research Council (CONICET), Buenos Aires, Argentina.

出版信息

Int J Parasitol Parasites Wildl. 2025 Jul 9;27:101114. doi: 10.1016/j.ijppaw.2025.101114. eCollection 2025 Aug.

Abstract

spp. and are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars () are IH for (with wild and domestic canids as DH), (with humans and non-human primates as DH) and (with felids as DH). The aim of this study was to identify and characterize spp. and in the muscle tissue of wild boars hunted and/or consumed in Switzerland. DNA was extracted from muscle samples of 286 wild boars and tested by PCRs targeting the gene of spp., the mitochondrial cytochrome oxidase () gene of and by a qPCR targeting a 529 bp genomic repeat of . . Besides, 225 samples were processed by homogenization and direct microscopic examination and 42 samples by histopathology. Sarcocysts were detected microscopically in 89.3 % (201/225) and 35.7 % (15/42) of the samples, respectively. The PCR detected parasite DNA in 91.3 % (261/286) of the samples, and all 62 sequences obtained were 100 % identical, and also with GenBank sequences reported as suggesting a high environmental contamination with canid faeces. The PCR was positive in 1.75 % of the samples (5/286), and the six sequences (772 bp primers trimmed) obtained were 97.3-99.6 % similar among them and 97.7-100 % identical with a sequence reported as . Despite the identity differences, all these sequences were grouped together in a phylogenetic tree, in a sister clade of sequences. All samples tested negative for by qPCR. This study revealed for the first time the presence of in wild boars hunted in Switzerland.

摘要

某些物种和……是异宿主原生动物寄生虫,它们在中间宿主(IH)的肌肉组织中形成包囊,在终末宿主(DH)的肠黏膜中形成卵囊。野猪(Sus scrofa)是某些物种的中间宿主(其终末宿主为野生和家养犬科动物)、……的中间宿主(其终末宿主为人类和非人灵长类动物)以及……的中间宿主(其终末宿主为猫科动物)。本研究的目的是鉴定和表征瑞士猎捕和/或食用的野猪肌肉组织中的某些物种和……。从286头野猪的肌肉样本中提取DNA,并通过针对某些物种的……基因的PCR、针对……的线粒体细胞色素c氧化酶(COX1)基因的PCR以及针对……的529 bp基因组重复序列的qPCR进行检测。此外,225个样本通过匀浆和直接显微镜检查进行处理,42个样本通过组织病理学进行处理。分别在89.3%(201/225)和35.7%(15/42)的样本中通过显微镜检测到包囊。……PCR在91.3%(261/286)的样本中检测到寄生虫DNA,获得的所有62个序列100%相同,并且与GenBank中报告为……的序列也相同,这表明犬科动物粪便对环境的污染程度很高。……PCR在1.75%的样本(5/286)中呈阳性,获得的六个……序列(去除772 bp引物)彼此之间的相似性为97.3 - 99.6%,与报告为……的一个序列的同一性为97.7 - 100%。尽管存在同一性差异,但所有这些序列在系统发育树中聚集在一起,位于……序列的一个姐妹分支中。所有样本通过qPCR检测……均为阴性。本研究首次揭示了在瑞士猎捕的野猪中存在……

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8441/12274798/e4db8b3d5cc6/ga1.jpg

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