Doerger P T, Glueck H I, van der Bel-Kahn J, Taylor A, Goldman L
Lasers Surg Med. 1985;5(5):457-68. doi: 10.1002/lsm.1900050504.
The effects of three lasers, Argon, Nd:YAG, and Argon-pumped Dye, on three types of platelet preparations were evaluated. Either EDTA or buffered citrate served as anticoagulants. Platelets separated from plasma and suspended in buffer showed no decrease in counts following lasering, but morphologic damage was evident with transmission electron microscopy (TEM). In platelet-rich plasma, a fall in counts was noted for the Argon and the YAG (at high energy only) but was absent when the Dye laser was employed. Morphologic damage (TEM), however, was noted with all three lasers. When whole blood preparations were used, more marked changes in both RBCs and platelets were seen in samples collected in EDTA compared with citrated samples. Morphologic damage (TEM) to platelets and RBCs occurred with all three lasers. An artifactual increase in "platelet counts," the appearance of spherocytes, and an increase in plasma Hb indicated RBC injury. Both platelets and erythrocytes were sensitive to variations in power (wattage) despite constant total energy delivery.
评估了氩离子激光、钕钇铝石榴石激光和氩离子泵浦染料激光这三种激光对三种血小板制剂的影响。乙二胺四乙酸（EDTA）或枸橼酸盐缓冲液用作抗凝剂。从血浆中分离并悬浮于缓冲液中的血小板在激光照射后计数未见减少，但透射电子显微镜（TEM）显示有明显的形态学损伤。在富血小板血浆中，氩离子激光和钕钇铝石榴石激光（仅在高能量时）照射后血小板计数下降，但使用染料激光时则无此现象。然而，所有三种激光均观察到形态学损伤（TEM）。当使用全血制剂时，与枸橼酸盐抗凝样本相比，乙二胺四乙酸抗凝样本中红细胞和血小板的变化更为明显。所有三种激光均导致血小板和红细胞出现形态学损伤（TEM）。“血小板计数”出现人为增加、球形红细胞出现以及血浆血红蛋白增加表明红细胞受到损伤。尽管总能量输送恒定，但血小板和红细胞均对功率（瓦数）变化敏感。
