2020年至2023年期间从中国台州腹泻患者粪便标本中分离出的菌株的特征分析。

Characterization of isolated from stool specimens of diarrhea patients in Taizhou, China during 2020-2023.

作者信息

Ding Ding, Jiang Yi, Lin Yajing, Zhang Ting, Guo Jiejie, Jiang Tian, Huang Linyao, Yan Jianxin, He Long

机构信息

Department of Laboratory, Affiliated Wenling Hospital of Wenzhou Medical University, Taizhou, ZheJiang, China.

Department of Laboratory, Wenling City Center for Disease Control and Prevention, Taizhou, ZheJiang, China.

出版信息

SAGE Open Med. 2025 Jul 17;13:20503121251355442. doi: 10.1177/20503121251355442. eCollection 2025.

DOI:10.1177/20503121251355442

PMID:40689262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12276508/

Abstract

PURPOSE

This study aimed to investigate the pathogenic characteristics of in Taizhou City, China, to provide a scientific basis for the diagnosis and control of in the region.

METHODS

Bacterial strains were isolated from stool specimens of diarrheal patients at the First People's Hospital of Wenling City. The virulence genes, serotypes, and antibiotic susceptibility of these strains were determined.

RESULTS

From June 1, 2020 to May 31, 2023, a total of 97 strains of were isolated from stool specimens of diarrheal patients at the First People's Hospital of Wenling City. The results of virulence gene detection showed that 87.6% of the strains were thermostable direct hemolysin (TDH; ), thermolabile hemolysin (), and -related hemolysin (), 11.3% were , and 1.0% were . Six different serotypes were identified, with O3:K4 and O4:K4 being the most common, accounting for 42.3% and 32.0%, respectively. The other serotypes identified were O3:K6, O4:K8, and O1:KUT, accounting for 11.3%, 10.3%, and 3.1%, respectively. Furthermore, the results of antimicrobial susceptibility tests showed that most strains were sensitive to the tested drugs, while some strains exhibited resistance to cefuroxime (32.6%) and cefazolin (43.6%).

CONCLUSION

The majority of the isolated strains carried the gene, and the O3:K4 serotype dominated in Taizhou City from 2020 to 2023, followed by the O4:K4 serotype. Furthermore, some strains exhibited resistance to cefuroxime and cefazolin.

摘要

目的

本研究旨在调查中国台州市[具体病原体名称未给出]的致病特征，为该地区[具体病原体名称未给出]的诊断和防控提供科学依据。

方法

从温岭市第一人民医院腹泻患者的粪便标本中分离细菌菌株。测定这些菌株的毒力基因、血清型和抗生素敏感性。

结果

2020年6月1日至2023年5月31日，温岭市第一人民医院腹泻患者的粪便标本中共分离出97株[具体病原体名称未给出]。毒力基因检测结果显示，87.6%的菌株携带耐热直接溶血素（TDH；[相关具体内容未给出]）、不耐热溶血素（[相关具体内容未给出]）和[相关溶血素名称未给出]溶血素（[相关具体内容未给出]），11.3%携带[相关基因名称未给出]，1.0%携带[相关基因名称未给出]。鉴定出六种不同血清型，其中O3:K4和O4:K4最为常见，分别占42.3%和32.0%。鉴定出的其他血清型为O3:K6、O4:K8和O1:KUT，分别占11.3%、10.3%和3.1%。此外，抗菌药物敏感性试验结果显示，大多数菌株对所测试药物敏感，而一些菌株对头孢呋辛（32.6%）和头孢唑林（43.6%）表现出耐药性。