Manzoor Tasaduq, Dar Lateef Ahmad, Mir Yaawar Bashir, Shah Showkat Ahmad, Ahmad Sheikh F, Godha Meena, Ahmad Syed Mudasir
Division of Animal Biotechnology, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, 190006, India.
School of Life and Basic Sciences, Jaipur National University, Jaipur, 302017, India.
J Mater Chem B. 2025 Aug 6;13(31):9486-9497. doi: 10.1039/d5tb00598a.
The development of advanced biomaterials with multifunctional properties is essential to address the complex challenges of impaired wound healing and tissue regeneration. This study introduces a novel composite scaffold (SSP-CG), in which silk sericin (SS) and polyvinyl alcohol (PVA) form the SSP component, while copper nanoparticles (CuNPs) and gallic acid (GA) constitute the CG component. SS provides biocompatibility and biodegradability, while PVA enhances structural integrity. CuNPs and GA impart antimicrobial and antioxidant activity, respectively, making the scaffold highly suitable for biomedical applications. The scaffold features an optimal pore size (96 ± 19 μm) and pore volume, promoting cell infiltration and nutrient diffusion. degradation studies revealed a controlled, sustained profile over 6 weeks, ideal for long-term therapeutic use. A gradual and prolonged release of GA ensured continuous antioxidant activity, confirmed by a DPPH assay showing significant free radical scavenging activity (40.5 ± 2.1%). studies further confirmed excellent biocompatibility, with optimal cell adhesion, proliferation, and viability while maintaining the environment for tissue regeneration. studies demonstrated superior wound healing outcomes for the SSP-CG scaffold compared to both positive and negative controls, with histological analysis further confirming enhanced tissue regeneration and reduced inflammation. This first-of-its-kind integration of SS, PVA, CuNPs, and GA highlights the synergistic benefits of these components, offering a promising solution for advanced wound healing and tissue regeneration. These findings suggest that SSP-CG scaffolds could contribute to next-generation biomaterials tailored for chronic wound management and regenerative therapies.
开发具有多功能特性的先进生物材料对于应对伤口愈合受损和组织再生的复杂挑战至关重要。本研究引入了一种新型复合支架（SSP-CG），其中丝胶蛋白（SS）和聚乙烯醇（PVA）形成SSP组分，而铜纳米颗粒（CuNPs）和没食子酸（GA）构成CG组分。SS提供生物相容性和生物降解性，而PVA增强结构完整性。CuNPs和GA分别赋予抗菌和抗氧化活性，使该支架非常适合生物医学应用。该支架具有最佳孔径（96±19μm）和孔体积，促进细胞浸润和营养物质扩散。降解研究表明，在6周内具有可控的、持续的降解过程，非常适合长期治疗使用。GA的逐渐和持续释放确保了持续的抗氧化活性，通过DPPH测定法证实具有显著的自由基清除活性（40.5±2.1%）。研究进一步证实了其优异的生物相容性，具有最佳的细胞粘附、增殖和活力，同时维持组织再生的环境。研究表明，与阳性和阴性对照相比，SSP-CG支架具有更好的伤口愈合效果，组织学分析进一步证实了组织再生增强和炎症减轻。这种首次将SS、PVA、CuNPs和GA整合在一起的方式突出了这些组分的协同优势，为先进的伤口愈合和组织再生提供了一个有前景的解决方案。这些发现表明，SSP-CG支架可为针对慢性伤口管理和再生疗法量身定制的下一代生物材料做出贡献。
