Lyu Hui, Tang Zhaoqi, Xu Weijie, Kong Dejun, Wang Zhihong, Yang Zhixiao, Zhang Jishun, Wu Hongqi, Wang Zili, Lazár Dušan, Lin Yingchao
School of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun, China.
Shanghai Tobacco Group Co., Ltd, Shanghai, China.
Plant Physiol Biochem. 2025 Jul 12;228:110239. doi: 10.1016/j.plaphy.2025.110239.
To explore the variation in adaptability to fluctuating light environments, the non-photochemical quenching (NPQ) characteristics of a local tobacco variety Xiaofeng 8 and a foreign introduced variety K326, under both constant and fluctuating light conditions were examined. The field experiment demonstrated that Xiaofeng 8 exhibited significantly stronger growth compared to K326, with the dry weight per plant and leaf area of Xiaofeng 8 increased by 28.26 % and 32.85 % compared with those of K326, respectively. Further, a distinct activation pattern of NPQ was detected in both varieties during the transition from dark to high light. Building on this finding, our subsequent tests proved that, in the high light intensity segments of a fluctuating light environment, Xiaofeng 8 exhibited a higher NPQ value compared to K326, indicating an enhanced capacity for dissipating excess light energy as thermal energy and mitigating the potential risk of photodamage. Subsequent analysis of the contents of violaxanthin (V), antheraxanthin (A), and zeaxanthin (Z) within the two varieties after high - light treatment has proved that Xiaofeng 8 has a superior ability to dissipate excess absorbed light energy under high - light conditions. This ability may contribute to its better growth under fluctuating light environments. Mutants defective in violaxanthin de-epoxidase (VDE) and zeaxanthin epoxidase (ZEP) were employed to examine their involvement in regulating plants growth under fluctuating light condition. The results showed that defects in these enzymes affected the NPQ response and reduced the growth rate of the mutants under fluctuating light conditions, suggesting its pivotal role of xanthophyll in regulating plant growth and adaptation to variable light environments. Collectively, these findings emphasize the importance of exploring genetic resources improving plant NPQ under fluctuating light conditions from both domestic and imported varieties, thereby providing promising targets for selecting and breeding new crop varieties with improved yield.
为探究对波动光环境适应性的变化，研究了本地烟草品种小峰8和引进品种K326在恒定光和波动光条件下的非光化学猝灭（NPQ）特性。田间试验表明，小峰8的生长显著强于K326，小峰8的单株干重和叶面积分别比K326增加了28.26%和32.85%。此外，在从黑暗到高光的转变过程中，两个品种均检测到明显的NPQ激活模式。基于这一发现，后续测试证明，在波动光环境的高光强度段，小峰8的NPQ值高于K326，这表明其将过剩光能以热能形式耗散以及减轻光损伤潜在风险的能力增强。高光处理后对两个品种中紫黄质（V）、花药黄质（A）和玉米黄质（Z）含量的后续分析证明，小峰8在高光条件下具有更强的过剩吸收光能耗散能力。这种能力可能有助于其在波动光环境下更好地生长。利用紫黄质脱环氧化酶（VDE）和玉米黄质环氧化酶（ZEP）缺陷的突变体来研究它们在波动光条件下对植物生长调节的参与情况。结果表明，这些酶的缺陷影响了NPQ响应，并降低了突变体在波动光条件下的生长速率，表明叶黄素在调节植物生长和适应可变光环境中起关键作用。总的来说，这些发现强调了从国内和引进品种中探索改善波动光条件下植物NPQ的遗传资源的重要性，从而为选育高产新作物品种提供有前景的目标。
