Jasinskiene N E, Jasinskas A L, Gineitis A A
Mol Biol Rep. 1985 Oct;10(4):199-203. doi: 10.1007/BF00775976.
Chromatin fractions differing in their transcriptional activity were isolated by selective micrococcal nuclease digestion of nuclei from sea urchin embryos (Strongylocentrotus droebachiensis) at the gastrula and pluteus stage. The electrophoretic analysis of the chromatin proteins at the gastrula stage showed that a soluble, transcriptionally active fraction of chromatin was enriched with early variants of histones H1 and H2A. The early and late variants of histone H2A at the pluteus stage were distributed randomly between chromatin fractions. However, the content of both variants of histone H1 was essentially decreased in the soluble transcriptionally active fraction of chromatin.
通过对海胆胚胎（强壮球海胆）原肠胚期和长腕幼虫期的细胞核进行选择性微球菌核酸酶消化，分离出转录活性不同的染色质组分。对原肠胚期染色质蛋白的电泳分析表明，染色质的可溶性转录活性组分富含组蛋白H1和H2A的早期变体。长腕幼虫期组蛋白H2A的早期和晚期变体随机分布在染色质组分之间。然而，染色质可溶性转录活性组分中组蛋白H1两种变体的含量均显著降低。
