Herlands L, Allfrey V G, Poccia D
J Cell Biol. 1982 Jul;94(1):219-23. doi: 10.1083/jcb.94.1.219.
The pattern and schedule of histone synthesis in unfertilized eggs and early embryos of the sea urchin Strongylocentrotus purpuratus were studied using two-dimensional gel electrophoresis. After fertilization there is an abrupt change in the pattern of histone variant synthesis. Although both cleavage-stage (CS) variants. However, after fertilization, both CS and alpha messages are translated. Since alpha histone mRNA isolated from unfertilized eggs can be translated in vitro, the synthesis of alpha histone subtypes appears to be under translational control. Although the synthesis of alpha subtypes is shown here to occur before the second S phase after fertilization, little or no alpha histone is incorporated into chromatin at this time. Thus, early chromatin is composed predominantly of CS variants probably recruited for the most part from the large pool of CS histones stored in the unfertilized egg.
利用双向凝胶电泳技术研究了紫海胆未受精卵和早期胚胎中组蛋白合成的模式和时间表。受精后，组蛋白变体合成模式发生突然变化。虽然卵裂期（CS）变体都有。然而，受精后，CS和α信息都被翻译。由于从未受精卵中分离出的α组蛋白mRNA可以在体外翻译，α组蛋白亚型的合成似乎受到翻译控制。虽然这里显示α亚型的合成发生在受精后的第二个S期之前，但此时很少或没有α组蛋白被整合到染色质中。因此，早期染色质主要由CS变体组成，这些变体可能大部分是从未受精卵中储存的大量CS组蛋白中募集而来的。
