利用双水相体系在盐溶液中解离和分离染色质蛋白。

Dissociation and isolation of chromatin proteins in salt solutions by an aqueous two-phase system.

作者信息

Gineitis A A, Suciliene S P, Shanbhag V P

出版信息

Anal Biochem. 1984 Jun;139(2):400-3. doi: 10.1016/0003-2697(84)90024-1.

Abstract

An aqueous two-phase system containing 7% Dextran T 500-5% polyethylene glycol (PEG) 6000 has been adopted for rapid selective stepwise extractions of high-mobility-group proteins and histones from both isolated chromatin and intact nuclei of calf thymus. After the dissociated proteins in the PEG phase were precipitated with 20% trichloroacetic acid at 4 degrees C, proteins were recovered from this phase by solubilization of PEG with acidified acetone at room temperature. This method allows preparation of nuclei depleted of histone H1.

摘要

含有7%葡聚糖T 500-5%聚乙二醇（PEG）6000的双水相系统已被用于从小牛胸腺的分离染色质和完整细胞核中快速选择性逐步提取高迁移率族蛋白和组蛋白。在4℃下用20%三氯乙酸沉淀PEG相中的解离蛋白后，通过在室温下用酸化丙酮溶解PEG从该相中回收蛋白质。该方法允许制备不含组蛋白H1的细胞核。