Zakharyan Roksana, Hakobyan Siras, Brojakowska Agnieszka, Bisserier Malik, Zhang Shihong, Khlgatian Mary K, Rai Amit Kumar, Davitavyan Suren, Stepanyan Ani, Sirunyan Tamara, Khachatryan Gisane, Sahoo Susmita, Garikipati Venkata Naga Srikanth, Arakelyan Arsen, Goukassian David A
Institute of Molecular Biology, National Academy of Science of Republic of Armenia, Yerevan, Armenia.
Russian-Armenian University, Yerevan, Armenia.
NPJ Microgravity. 2025 Jul 21;11(1):46. doi: 10.1038/s41526-025-00506-8.
Deep space represents a challenging environment for human exploration and can be accompanied by harmful health-related risks. We aimed to assess the effect of simplified galactic cosmic ray simulated (simGCRsim) and gamma (γ) ionizing radiation (IR) on transcriptome changes in right ventricular (RV) tissue after a single low dose (0.5 Gy, 500 MeV/nucleon) full body exposure in C57BL/6J male and female mice. In females, no differentially expressed genes (DEGs) and only 2 upregulated genes in males exposed to γ-IR were revealed. In contrast, exposure to simGCRsim-IR resulted in 4 DEGs in females and 371 DEGs in males, suggesting longer-lasting and sex-biased DEGs after simGCRsim-IR. Overrepresentation analysis of DEGs in simGCRsim-IR males revealed significant enrichment in pathways related to muscle contraction, hypertrophic cardiomyopathy, oxytocin release, the regulation of cytoskeleton, and genes associated with Alzheimer's, Huntington's, and Parkinson's diseases. Our results suggested the RV transcriptome exhibits distinct responses after exposure based on both the IR and sex.
深空对人类探索而言是一个充满挑战的环境，并且可能伴随着与健康相关的有害风险。我们旨在评估在C57BL/6J雄性和雌性小鼠单次低剂量（0.5 Gy，500 MeV/核子）全身照射后，简化的银河宇宙射线模拟（simGCRsim）和伽马（γ）电离辐射（IR）对右心室（RV）组织转录组变化的影响。在雌性小鼠中，未发现差异表达基因（DEG），而在接受γ-IR照射的雄性小鼠中仅发现2个上调基因。相比之下，暴露于simGCRsim-IR导致雌性小鼠中有4个DEG，雄性小鼠中有371个DEG，这表明simGCRsim-IR后存在更持久且具有性别偏向的DEG。对simGCRsim-IR雄性小鼠中DEG的过度表达分析显示，与肌肉收缩、肥厚性心肌病、催产素释放、细胞骨架调节以及与阿尔茨海默病、亨廷顿病和帕金森病相关的基因有关的通路显著富集。我们的结果表明，基于辐射类型和性别，右心室转录组在暴露后表现出不同的反应。
