Nakahashi Naoya, Emori Makoto, Murahashi Yasutaka, Murase Kazuyuki, Shimizu Junya, Murata Kenji, Tsukahara Tomohide, Sugita Shintaro, Iba Kousuke, Takada Kohichi, Osanai Makoto, Teramoto Atsushi
Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, West 16, South 1, Chuo- Ku, Sapporo, 060-8543, Japan.
Department of Medical Oncology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Hum Cell. 2025 Jul 22;38(5):132. doi: 10.1007/s13577-025-01263-2.
Pleomorphic rhabdomyosarcoma (pRMS) is a rare and highly malignant mesenchymal tumor. Complete resection is the only curative treatment available, owing to the limited efficacy of chemotherapy and radiotherapy. Therefore, developing novel therapies for pRMS is important for improving clinical outcomes. Herein, a novel pRMS cell line, SMU-pRMS, was established for a detailed understanding of the biological characteristics of pRMS, thereby developing new therapies. A tissue sample from a surgically resected tumor of a 69-year-old patient was subjected to primary culture. The cell line was established and authenticated by evaluating the short tandem repeats of DNA microsatellites. Monolayer cultures of SMU-pRMS cells exhibited constant growth, spheroid formation, and invasiveness. These cells exhibited high chemosensitivity to eribulin. In addition, mice inoculated with SMU-pRMS cells developed tumors after 4 weeks. Therefore, the SMU-pRMS cell line is a useful tool for investigating pRMS development and evaluating novel therapeutic agents.
多形性横纹肌肉瘤（pRMS）是一种罕见的高度恶性间充质肿瘤。由于化疗和放疗效果有限，完整切除是唯一可用的治愈性治疗方法。因此，开发针对pRMS的新疗法对于改善临床结果至关重要。在此，建立了一种新型pRMS细胞系SMU-pRMS，以详细了解pRMS的生物学特性，从而开发新疗法。对一名69岁患者手术切除肿瘤的组织样本进行原代培养。通过评估DNA微卫星的短串联重复序列建立并鉴定了该细胞系。SMU-pRMS细胞的单层培养物表现出持续生长、球体形成和侵袭性。这些细胞对艾日布林表现出高化学敏感性。此外，接种SMU-pRMS细胞的小鼠在4周后出现肿瘤。因此，SMU-pRMS细胞系是研究pRMS发展和评估新型治疗药物的有用工具。
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