Wodniok Wiktoria
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Katowice, Poland.
Quant Plant Biol. 2025 Jul 18;6:e18. doi: 10.1017/qpb.2025.10009. eCollection 2025.
Organ morphogenesis is a complex process and numerous factors must be considered while choosing a method for its quantitative investigation. Few methods facilitate imaging. These are sequential replica methods combined with scanning electron microscopy and sequential confocal microscopy imaging. The latter is now the most used method to study spatiotemporal changes of organ geometry, growth and involvement of molecular factors in regulating organ development. The time-lapse confocal imaging combined with quantitative analysis of the spatiotemporal pattern of auxin efflux proteins (PIN-FORMED) was used to investigate growth and morphogenesis of gynoecium and enabled detailed insight into gynoecium development. Yet time-lapse imaging of the gynoecium, concealed within a flower bud, presents challenges in ensuring high-quality data during all the stages of such investigations (sample preparation, maintenance of growing organ during the relatively long time of its development, laser exposure time, etc.). Analysis of vast quantitative data was facilitated by MorphoGraphX.
器官形态发生是一个复杂的过程，在选择定量研究方法时必须考虑众多因素。很少有方法便于成像。这些方法是与扫描电子显微镜相结合的连续复制方法以及连续共聚焦显微镜成像。后者是目前研究器官几何形状的时空变化、生长以及分子因素参与调节器官发育最常用的方法。延时共聚焦成像结合生长素流出蛋白（PIN-FORMED）时空模式的定量分析被用于研究雌蕊的生长和形态发生，并能够深入了解雌蕊发育。然而，隐藏在花芽内的雌蕊的延时成像在确保此类研究的所有阶段（样品制备、在其发育的相对较长时间内维持生长器官、激光照射时间等）获得高质量数据方面存在挑战。MorphoGraphX有助于对大量定量数据进行分析。
