Scisłowski P W, Słomiński A, Bomirski A, Zydowo M
Neoplasma. 1985;32(5):593-8.
Some enzyme activities and metabolic features of the black Ma melanotic, brown MI melanotic and Ab amelanotic melanomas of hamster were investigated. The activities of hexokinase and phosphofructokinase were similar in all three melanomas, the activity of NAD-dependent glycerol-3-phosphate dehydrogenase was higher in the amelanotic melanoma and that of pyruvate kinase and lactate dehydrogenase were slightly lower in MI than in the other tumors. The activities of citrate synthase, succinate dehydrogenase and malate dehydrogenase were higher in the Ma and MI melanotic melanomas than in the Ab amelanotic melanoma. The rate of labeled CO2 production from 6-14C-glucose, 1,5-14C-citric acid and U-14C-glutamine was about 2 times higher in melanotic melanomas than in amelanotic one, while no significant differences among the three melanomas were found in respect to 1-14C-glucose and U-14C-glycerol-3-phosphate. The production of 14CO2 was much higher from 1-14C-glucose than from 6-14C-glucose in all the melanomas studied. L-DOPA stimulated the production of 14CO2 from 1-14C-glucose much stronger in the Ma and MI melanomas than in the Ab melanoma. In none of the tumors the incorporation from 6-14C-glucose to CO2 was affected by L-DOPA. It is postulated that oxidation of glucose via the pentose phosphate cycle is involved in melanogenesis.
对仓鼠的黑色Ma黑色素瘤、棕色MI黑色素瘤和无黑色素的Ab黑色素瘤的一些酶活性和代谢特征进行了研究。己糖激酶和磷酸果糖激酶的活性在所有三种黑色素瘤中相似，NAD依赖的甘油-3-磷酸脱氢酶的活性在无黑色素的黑色素瘤中较高，丙酮酸激酶和乳酸脱氢酶的活性在MI中比在其他肿瘤中略低。柠檬酸合酶、琥珀酸脱氢酶和苹果酸脱氢酶的活性在Ma和MI黑色素瘤中高于Ab无黑色素的黑色素瘤。来自6-14C-葡萄糖、1,5-14C-柠檬酸和U-14C-谷氨酰胺的标记CO2产生率在黑色素瘤中比在无黑色素的黑色素瘤中高约2倍，而在1-14C-葡萄糖和U-14C-甘油-3-磷酸方面，三种黑色素瘤之间未发现显著差异。在所有研究的黑色素瘤中，1-14C-葡萄糖产生的14CO2比6-14C-葡萄糖产生的14CO2要高得多。L-DOPA在Ma和MI黑色素瘤中比在Ab黑色素瘤中更强烈地刺激1-14C-葡萄糖产生14CO2。在所有肿瘤中，L-DOPA均未影响6-14C-葡萄糖向CO2的掺入。据推测，通过磷酸戊糖途径氧化葡萄糖参与了黑色素生成。
