Demirci Kayıran Serpil, Tavlı Ömerül Faruk, Mataracı Kara Emel, Kaplan Alevcan, Şahin Hasan, Boğa Mehmet, Eroğlu Özkan Esra
Department of Pharmaceutical Botany, Faculty of Pharmacy, Çukurova University, Adana, Türkiye.
Department of Pharmacognosy, Faculty of Pharmacy, Biruni University, Istanbul, Türkiye.
Front Pharmacol. 2025 Jul 7;16:1618761. doi: 10.3389/fphar.2025.1618761. eCollection 2025.
subsp. Willd. (Hypericaceae), traditionally used in folk medicine, was investigated for its diverse biological activities. The study was driven by the increasing global health and economic burden posed by fungal infections, highlighting the urgent need for novel antifungal agents.
Plant materials were collected from distinct regions in Türkiye. Methanolic extracts were prepared and tested in vitro for antimicrobial and enzyme inhibition activities. Minimum inhibitory concentration (MIC) and IC values were determined using standard microdilution and spectrophotometric assays. Phytochemical profiling of the extracts was performed using HPLC-DAD and LC-HR/MS to identify major chemical constituents and investigate variation due to geographic and environmental factors.
Methanolic extracts exhibited potent antifungal activity against strains, with MIC values as low as 4.88 μg/mL. Enzyme inhibition assays revealed strong activity for HE-1, with IC values of 8.16 μg/mL for acetylcholinesterase and 2.46 μg/mL for butyrylcholinesterase. The extracts also significantly inhibited α-glucosidase (IC ≈ 26-31 μg/mL), outperforming acarbose, and showed moderate inhibition against elastase. Phytochemical profiling indicated notable variations in flavonoid and phenolic acid content, likely influenced by geographic and environmental factors.
These findings suggest that subsp. extracts possess promising antifungal, antidiabetic, anti-aging, and neuroprotective properties. The observed bioactivities and phytochemical richness support further exploration of this species as a potential source of therapeutic agents.
Willd.亚种（金丝桃科），传统上用于民间医学，对其多种生物活性进行了研究。该研究是由真菌感染给全球健康和经济带来的日益沉重的负担所推动的，凸显了对新型抗真菌药物的迫切需求。
从土耳其不同地区采集植物材料。制备甲醇提取物并在体外测试其抗菌和酶抑制活性。使用标准微量稀释法和分光光度法测定最低抑菌浓度（MIC）和IC值。使用HPLC-DAD和LC-HR/MS对提取物进行植物化学分析，以鉴定主要化学成分并研究地理和环境因素导致的差异。
甲醇提取物对菌株表现出强大的抗真菌活性，MIC值低至4.88μg/mL。酶抑制试验显示HE-1具有较强活性，对乙酰胆碱酯酶的IC值为8.16μg/mL，对丁酰胆碱酯酶的IC值为2.46μg/mL。提取物还显著抑制α-葡萄糖苷酶（IC≈26 - 31μg/mL），优于阿卡波糖，并对弹性蛋白酶表现出中等抑制作用。植物化学分析表明黄酮类和酚酸含量存在显著差异，可能受地理和环境因素影响。
这些发现表明Willd.亚种提取物具有有前景的抗真菌、抗糖尿病、抗衰老和神经保护特性。观察到的生物活性和植物化学丰富性支持进一步探索该物种作为治疗药物的潜在来源。
