Lepetit P, Lestage P, Gauquelin G, Vitte P A, Debilly G, Gharib C, Jouvet M, Bobillier P
Neurosci Lett. 1985 Nov 20;62(1):13-8. doi: 10.1016/0304-3940(85)90277-0.
The rate of methionine incorporation was measured in individual hypothalamic nuclei by a quantitative autoradiographic technique: the L-[35S]methionine method. Physiological stimulation of the hypothalamo-neurohypophysial system by two days of water deprivation, a stress that increased the plasma total neurophysins concentration 4-5-fold, resulted in a 70-80% increase in overall protein synthesis in the supraoptic nucleus and in the magnocellular subdivisions of the paraventricular nucleus. The same stimulus had no effect on protein synthesis rate of the suprachiasmatic nucleus and of the other representative cerebral structures examined. This differential effect on protein synthesis was also observed within the paraventricular nucleus.
采用定量放射自显影技术（L-[35S]蛋氨酸法）测定了各个下丘脑核团中蛋氨酸的掺入率。通过两天禁水对下丘脑-神经垂体系统进行生理刺激，这种应激使血浆中总神经垂体素浓度增加了4至5倍，结果视上核和室旁核大细胞亚群中的总体蛋白质合成增加了70%至80%。相同的刺激对视交叉上核以及所检查的其他代表性脑结构的蛋白质合成速率没有影响。在室旁核内也观察到了对蛋白质合成的这种差异效应。
