Bandaranayake R C
Acta Anat (Basel). 1976;95(3):408-20. doi: 10.1159/000144629.
Nucleo-cytoplasmic transfer rates of RNA were used as indices of protein-synthetic activity in mammalian supraoptic and paraventricular neurones. Normal, dehydrated and lactating rats were exposed, for varying periods of time, to tritiated uridine, and autoradiographs obtained from hypothalamic blocks. Grain count and cytometry enabled a cytoplasmic/nuclear ratio of grain densities to be obtained for each neurosecretory nucleus. Comparison of these ratios, as well as the regression slopes for their increase with time of exposure to the isotope, indicated the presence of a differential response between the supraoptic and paraventricular nuclei, with the former responding more to the stimulus of dehydration, and the latter to that of lactation. The observations substantiate the view that a partial division of labour exists between the two nuclei in the elaboration of each of the mammalian neurohypophysial octapeptides, vasopressin and oxytocin.
RNA的核质转运速率被用作哺乳动物视上核和室旁核中蛋白质合成活性的指标。将正常、脱水和哺乳期大鼠在不同时间段暴露于氚标记的尿苷，并从下丘脑切片获得放射自显影片。通过颗粒计数和细胞计数法能够得出每个神经分泌核的颗粒密度的细胞质/核比率。比较这些比率以及它们随同位素暴露时间增加的回归斜率，表明视上核和室旁核之间存在差异反应，前者对脱水刺激反应更强，后者对泌乳刺激反应更强。这些观察结果证实了这样一种观点，即在哺乳动物神经垂体八肽（抗利尿激素和催产素）的合成过程中，这两个核之间存在部分分工。
