Xia Xue, Dong Jiawei, Li Aijie, Wang Yanlin, Liu Yang, Zhu Yingfang, Xu Liang, Jing Zhiyang, Wang Jing, Zou Yan, Sun Shiyong, Wang Lu, Lu Yiqing, Soeriyadi Alex, Wang Xuelu, Patrick John W, Offler Christina E, Zheng Meng, Song Chun-Peng, Shi Bingyang
The Zhongzhou Laboratory for Integrative Biology, Henan-Macquarie University Joint Centre for Biomedical Innovation, School of Life Sciences, Henan University, Kaifeng, China.
Henan Key Laboratory of Brain Targeted Bio-nanomedicine, School of Life Sciences & School of Pharmacy, Henan University, Kaifeng, China.
Nat Commun. 2025 Jul 21;16(1):6715. doi: 10.1038/s41467-025-60829-8.
Presence of the cell wall and the lack of streamlined pathways for cellular delivery of external agents into plants is a core challenge of plant biotechnology and crop engineering development. However, both viral and bacterial transmission have their own restrictions and the few non-heavy metal nanodelivery platforms require external forces for tissue penetration. Such dependency limits any high-throughput application considering the large plant numbers to be treated in the field or even laboratory exercises. Herein, we demonstrate Aspartic acid (Asp) decorated poly(ethylene glycol)-block-poly(2-(diisopropylamino)ethyl methacrylate) (Asp-PEG-PDPA) copolymers assembled micelles (Asp/PDPA-NP), a platform that utilises amino acid transporters (AtAAP1 and AtLHT1) as receptors for clathrin-dependent endocytosis, freely translocate to release loaded cargo into various plant tissue/cell types in a species-independent manner within ≤10 minutes through simple spray or co-culture. As proof-of-concept, abscisic acid (ABA)-loaded Asp/PDPA-NP was tested for its efficacy to confer plant drought resistance. Asp/PDPA-NP@ABA reduced the effective ABA dose down to 1 nM (one million-fold) and elicited anti-drought potency in representative eudicot (soybean) and monocot (maize) crop species. Owing to its delivery efficiency, Asp/PDPA-NP holds promise as a potent carrier for diverse chemicals and biomolecules in plant systems.
细胞壁的存在以及缺乏将外部试剂高效递送至植物细胞内的流线型途径，是植物生物技术和作物工程发展的核心挑战。然而，病毒和细菌传播都有其自身的局限性，并且少数非重金属纳米递送平台需要外力来穿透组织。考虑到在田间甚至实验室操作中需要处理大量植物，这种依赖性限制了任何高通量应用。在此，我们展示了天冬氨酸（Asp）修饰的聚（乙二醇）-嵌段-聚（甲基丙烯酸2-（二异丙基氨基）乙酯）（Asp-PEG-PDPA）共聚物组装的胶束（Asp/PDPA-NP），该平台利用氨基酸转运蛋白（AtAAP1和AtLHT1）作为网格蛋白依赖性内吞作用的受体，通过简单的喷雾或共培养，在≤10分钟内以物种独立的方式自由转运以将负载的货物释放到各种植物组织/细胞类型中。作为概念验证，测试了负载脱落酸（ABA）的Asp/PDPA-NP赋予植物抗旱性的功效。Asp/PDPA-NP@ABA将有效的ABA剂量降低至1 nM（百万分之一），并在代表性的双子叶植物（大豆）和单子叶植物（玉米）作物品种中引发抗旱能力。由于其递送效率，Asp/PDPA-NP有望成为植物系统中多种化学物质和生物分子的有效载体。
