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微小RNA-370-3p通过靶向INO80影响绝经后骨质疏松症的进展。

miR-370-3p affects the progression of postmenopausal osteoporosis through targeting INO80.

作者信息

Yang Zhen, Sheng Yuqi, Liu Xiangjie, Cen Meini, Xu Yong

机构信息

Hepatic Department, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.

School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, China.

出版信息

Hereditas. 2025 Jul 22;162(1):138. doi: 10.1186/s41065-025-00502-8.

Abstract

BACKGROUND

Postmenopausal osteoporosis (PMO) is acknowledged as a principal category of osteoporosis (OP). The aim of this study was to investigate the level of miR-370-3p in PMO patients and its predictive effect on osteoporosis in postmenopausal women, and to explore the molecular mechanism of miR-370-3p on PMO.

METHODS

The expression of miR-370-3p was assessed using RT-qPCR. Cell proliferation of MC3T3-E1 cells was evaluated through CCK-8 assays. Cell apoptosis was detected by flow cytometry. The direct interaction between miR-370-3p and INO80 was confirmed via dual-luciferase reporter assays.

RESULTS

The level of miR-370-3p was found to be upregulated in osteoporosis patients, and miR-370-3p played a significant role in regulating the proliferation, apoptosis and differentiation of osteoblasts. In addition, miR-370-3p targeted INO80 and affected the disease progression of PMO.

CONCLUSIONS

miR-370-3p/INO80 may serve as a promising biomarker for both the diagnosis and therapeutic management of PMO.

摘要

背景

绝经后骨质疏松症(PMO)被认为是骨质疏松症(OP)的主要类型。本研究旨在调查PMO患者中miR-370-3p的水平及其对绝经后女性骨质疏松症的预测作用,并探讨miR-370-3p对PMO的分子机制。

方法

采用RT-qPCR评估miR-370-3p的表达。通过CCK-8试验评估MC3T3-E1细胞的细胞增殖。通过流式细胞术检测细胞凋亡。通过双荧光素酶报告试验证实miR-370-3p与INO80之间的直接相互作用。

结果

发现骨质疏松症患者中miR-370-3p水平上调,并且miR-370-3p在调节成骨细胞的增殖、凋亡和分化中起重要作用。此外,miR-370-3p靶向INO80并影响PMO的疾病进展。

结论

miR-370-3p/INO80可能作为PMO诊断和治疗管理的有前景的生物标志物。

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