Raguvaran Krishnan, Kalpana Manickam, Devapriya Palanisamy, Kalaivani Suresh, Angelina Marissa, Krismastuti Fransiska Sri Herwahyu, Rasool Akhtar, Maheswaran Rajan
Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Kompleks Puspitek Serpong, South Tangerang, Indonesia.
Entomology Laboratory, Department of Zoology, Periyar University, Salem, Tamil Nadu, India.
Luminescence. 2025 Jul;40(7):e70266. doi: 10.1002/bio.70266.
The increasing resistance of mosquitoes to synthetic pesticides necessitates eco-friendly alternatives for vector control. This study presents the green synthesis of silver nanoparticles (Ag-NPs) using Aerva lanata flower extract and evaluates their larvicidal efficacy against Cx. quinquefasciatus, An. stephensi, and Ae. aegypti. The biosynthesized Ag-NPs, characterized by UV-Vis, FT-IR, XRD, SEM-EDX, TEM, Zeta potential, and DLS analyses, exhibited potent larvicidal and pupicidal activities, with LC values of 6.501, 20.875, and 22.649 ppm, respectively. The morphological and histopathological alterations in treated larvae, alongside significant modulation of antioxidant (SOD, GPx) and detoxification (GST, AChE) enzymes, indicate oxidative stress as a key mode of action. Importantly, biosafety tests revealed minimal toxicity to Artemia salina, highlighting the eco-compatibility of these Ag-NPs. This study demonstrates an innovative, plant-based nanobiopesticide with strong potential for sustainable mosquito control, addressing the challenges of insecticide resistance and environmental toxicity.
蚊子对合成杀虫剂的抗性不断增强，因此需要采用生态友好型替代品来进行病媒控制。本研究介绍了使用白花艾草花提取物绿色合成银纳米颗粒（Ag-NPs）的方法，并评估了其对致倦库蚊、斯氏按蚊和埃及伊蚊的杀幼虫效果。通过紫外可见光谱、傅里叶变换红外光谱、X射线衍射、扫描电子显微镜-能谱分析、透射电子显微镜、zeta电位和动态光散射分析对生物合成的Ag-NPs进行表征，结果显示其具有强大的杀幼虫和杀蛹活性，LC值分别为6.501、20.875和22.649 ppm。处理后幼虫的形态和组织病理学变化，以及抗氧化酶（超氧化物歧化酶、谷胱甘肽过氧化物酶）和解毒酶（谷胱甘肽S-转移酶、乙酰胆碱酯酶）的显著调节，表明氧化应激是其关键作用模式。重要的是，生物安全性测试显示对卤虫的毒性极小，突出了这些Ag-NPs的生态兼容性。本研究展示了一种创新的、基于植物的纳米生物农药，在可持续控制蚊子方面具有强大潜力，可应对杀虫剂抗性和环境毒性等挑战。
