Hanka L J
Cancer Treat Rep. 1977 Jul;61(4):591-5.
The biochemical considerations applicable in the detection of new antimetabolites are very similar to those used in developing microbiologic assays for these drugs. An in vitro system for detection of antimetabolites was developed based on different sensitivities to such drugs of bacteria cultivated in complex versus completely synthetic media. A number of new antimetabolites of amino acids, vitamins, and purines or pyrimidines were isolated and microbiologic assays for these drugs were developed. As an example of the technology utilized and possibilities available, an assay for cytosine arabinoside was described. It can detect 0.5-microgram/ml concentrations of this drug even in the presence of other antitumor or antibacterial antibiotics.
适用于新型抗代谢物检测的生化考量与用于开发这些药物微生物检测方法的考量非常相似。基于在复杂培养基与完全合成培养基中培养的细菌对这类药物的不同敏感性，开发了一种体外抗代谢物检测系统。分离出了多种氨基酸、维生素以及嘌呤或嘧啶的新型抗代谢物，并开发了针对这些药物的微生物检测方法。作为所采用技术及可用可能性的一个示例，描述了一种阿糖胞苷检测方法。即使存在其他抗肿瘤或抗菌抗生素，该方法也能检测到浓度为0.5微克/毫升的这种药物。
