葵子麝香及其他硝基麝香化合物的薄层色谱法和高压液相色谱法，包括光斑贴试验研究。

Thin layer chromatography and high pressure liquid chromatography of musk ambrette and other nitromusk compounds including photopatch studies.

作者信息

Bruze M, Edman B, Niklasson B, Möller H

出版信息

Photodermatol. 1985 Oct;2(5):295-302.

Abstract

For qualitative and quantitative analysis of musk ambrette and 4 other nitromusk compounds (musk ketone, moskene, musk tibetine, musk xylene) thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC) techniques were developed. By TLC a reasonable separation was obtained and the limit of detection was 2-5 x 10(-7) g. By HPLC the separation was even better and the limit of detection for musk ambrette was 2 x 10(-9) g. The correlation between the amount of musk ambrette/ketone and the HPLC peak was linear. The TLC and HPLC techniques were used to demonstrate the presence of nitromusks in several commercial products, mainly aftershave lotions and eau-de-toilettes preparations. By ultraviolet spectrophotometry, absorption spectra were studied for the nitromusk compounds. The absorption maximum for musk ambrette was at 264 nm, that for moskene at 253 nm. Photopatch testing was carried out in 13 patients photoallergic to musk ambrette. Only 3 patients also reacted to other nitromusks. Photoallergey to musk ketone and musk tibetine is reported for the first time.

摘要

为了对葵子麝香及其他4种硝基麝香化合物（麝香酮、西藏麝香、吐纳麝香、二甲苯麝香）进行定性和定量分析，开发了薄层色谱法（TLC）和高压液相色谱法（HPLC）。通过TLC获得了合理的分离效果，检测限为2 - 5×10⁻⁷ g。通过HPLC分离效果更好，葵子麝香的检测限为2×10⁻⁹ g。葵子麝香/酮的含量与HPLC峰之间呈线性相关。TLC和HPLC技术主要用于证明几种商业产品中硝基麝香的存在，这些产品主要是须后水和淡香水制剂。通过紫外分光光度法，研究了硝基麝香化合物的吸收光谱。葵子麝香的最大吸收波长为264 nm，西藏麝香为253 nm。对13例对葵子麝香光过敏的患者进行了光斑贴试验。只有3例患者也对其他硝基麝香有反应。首次报道了对麝香酮和吐纳麝香的光过敏反应。