Araújo Rita, Guerra-Gomes Maria, Barros Joana, Gomes Pedro
BoneLab, Faculdade de Medicina Dentária, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal.
LAQV/REQUIMTE, Faculdade de Medicina Dentária, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal.
Methods Protoc. 2025 Jul 10;8(4):79. doi: 10.3390/mps8040079.
Burn wounds present a significant challenge to both the medical and scientific communities, contributing to the global economic burden on healthcare systems. Due to the complexity and highly variability of burn injuries, along with intricate pathophysiological mechanisms, the development of appropriate and effective treatment strategies remains particularly demanding. The development of robust pre-clinical models that recapitulate specific molecular and cellular events underlying burn injury are essential to advance the understanding of associated biological mechanisms and facilitate the screening of innovative therapeutic interventions. While conventional in vivo models can replicate the key aspects of human burn wound pathology, they are often associated with ethical, logistical, and cost-related limitations. In this context, this study aims to explore the potential of the chicken chorioallantoic membrane (CAM) as an alternative model for burn wound research. Thus, we describe a reproducible and ethically favorable protocol for establishing standardized burn injuries on the CAM and provide a comprehensive evaluation of tissue responses through macroscopic, morphometric, and histological analyses. Our findings support the CAM as a viable pre-clinical platform for the study of burn wound healing and for the early-stage screening of candidate therapeutic agents.
烧伤创面给医学和科学界带来了重大挑战，加重了全球医疗系统的经济负担。由于烧伤损伤的复杂性和高度变异性，以及复杂的病理生理机制，开发合适且有效的治疗策略仍然极具挑战性。建立能够重现烧伤损伤潜在特定分子和细胞事件的强大临床前模型，对于增进对相关生物学机制的理解以及促进创新治疗干预措施的筛选至关重要。虽然传统的体内模型可以复制人类烧伤创面病理学的关键方面，但它们往往存在伦理、后勤和成本相关的限制。在此背景下，本研究旨在探索鸡胚绒毛尿囊膜（CAM）作为烧伤创面研究替代模型的潜力。因此，我们描述了一种可重复且符合伦理的方案，用于在CAM上建立标准化烧伤损伤，并通过宏观、形态计量和组织学分析对组织反应进行全面评估。我们的研究结果支持CAM作为研究烧伤创面愈合和早期筛选候选治疗药物的可行临床前平台。
