一种编码60聚体尼帕病毒G糖蛋白纳米颗粒的mRNA疫苗引发了针对尼帕病毒的强大中和抗体反应。

A mRNA vaccine encoding for a 60-mer Nipah virus G glycoprotein nanoparticle elicits a robust neutralizing antibodies response against the Nipah virus.

作者信息

Brandys Pascal, Albariño César G, Jain Shilpi, Merenkova Irena, Schork Nicholas J, Deng Aihua, Valière Martine, Herold Jens

机构信息

Phylex BioSciences, Del Mar, CA, United States.

Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA, United States.

出版信息

Vaccine. 2025 Jul 22;62:127530. doi: 10.1016/j.vaccine.2025.127530.

DOI:10.1016/j.vaccine.2025.127530

PMID:40700900

Abstract

The Nipah virus is a zoonotic pathogen causing encephalitis and acute respiratory illness in humans with very high fatality rates. Here we report a novel messenger RNA vaccine that directly encodes for a nanoparticle displaying 60 head domains of the Nipah virus G (NiV G) glycoprotein that acts as a highly effective antigen. A vaccine encoding for the NiV G nanoparticle elicits high antibody titers against NiV G and a robust neutralizing antibody response with a pseudotyped Nipah virus system. We ultimately find that the proposed mRNA NiV G nanoparticle (mRNA NiV G-NP) provides a flexible platform for the development of vaccines that will likely be of great value in combatting future Nipah virus outbreaks.

摘要

尼帕病毒是一种人畜共患病原体，可导致人类患脑炎和急性呼吸道疾病，病死率极高。在此，我们报告一种新型信使核糖核酸疫苗，该疫苗直接编码一种展示尼帕病毒G（NiV G）糖蛋白60个头部结构域的纳米颗粒，该纳米颗粒作为一种高效抗原。编码NiV G纳米颗粒的疫苗可引发针对NiV G的高抗体滴度，并通过假型尼帕病毒系统产生强大的中和抗体反应。我们最终发现，所提出的信使核糖核酸NiV G纳米颗粒（mRNA NiV G-NP）为疫苗开发提供了一个灵活的平台，这可能对对抗未来的尼帕病毒爆发具有巨大价值。