Câmara Neto João Francisco, Campelo Matheus da Silva, da Costa Mac Dionys Rodrigues, Pinheiro Natasha Maria Lima, Ricardo Nágila Maria Pontes Silva, Uchoa Daniel Esdras de Andrade, Cerqueira Gilberto Santos, Sampaio Tiago Lima, Ribeiro Maria Elenir Nobre Pinho
Department of Organic and Inorganic Chemistry, Science Center, Federal University of Ceará, Fortaleza, CE, ZIP code 60440-900, Brazil.
Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Dentistry and Nursing, Fortaleza, CE, ZIP code 60430-160, Brazil.
Int J Biol Macromol. 2025 Sep;321(Pt 2):146170. doi: 10.1016/j.ijbiomac.2025.146170. Epub 2025 Jul 21.
This study aims to isolate and characterize the physicochemical properties and evaluate the cytoprotective and gastroprotective potential of polysaccharides from the Agaricus blazei Murill mushroom (PAbM). The PAbM were isolated and characterized chemically. The cytoprotective potential of PAbM was studied using LLC-MK2 cells through the acetic acid-induced cell injury model. The gastroprotective activity was studied using the ethanol-induced gastric ulcer model. The PAbM showed a molecular mass of 4.7 × 105 g mol and polydisperse character, being composed by glucose, mannose and galactose monomers. In vitro study showed the low cytotoxicity of PAbM against LLC-MK2 cells and their ability to attenuate cellular injury (3.12 and 6.25 μmol/L) caused by exposure to acetic acid, being able to reduce the parameters related to oxidative stress and prevent the occurrence of cellular alterations. The in vivo assay demonstrated the ability of PAbM (50 and 150 mg/kg, p.o.) to alleviate the morphological changes in the gastric mucosa induced by the administration of ethanol. PAbM were able to reduce tissue mastocytosis and these results were more promising than those obtained for reference drug (carbenoxolone at 200 mg/kg, p.o.). The administration of PAbM was able to reduce TBARS levels and increase GSH levels, evidencing their in vivo antioxidant activity. Thus, this study reports the gastroprotective potential of PAbM and encourages future studies in the preclinical context to better understand the mechanisms linked to this activity and their use in clinical trials aimed at a new treatment for gastric ulcer.
本研究旨在分离并表征姬松茸多糖（PAbM）的理化性质，评估其细胞保护和胃保护潜力。对PAbM进行了化学分离和表征。通过醋酸诱导的细胞损伤模型，利用LLC-MK2细胞研究了PAbM的细胞保护潜力。利用乙醇诱导的胃溃疡模型研究了其胃保护活性。PAbM的分子量为4.7×105 g/mol，具有多分散性，由葡萄糖、甘露糖和半乳糖单体组成。体外研究表明，PAbM对LLC-MK2细胞的细胞毒性较低，并且能够减轻因接触醋酸引起的细胞损伤（3.12和6.25 μmol/L），能够降低与氧化应激相关的参数并防止细胞改变的发生。体内试验表明，PAbM（50和150 mg/kg，口服）能够减轻乙醇给药诱导的胃黏膜形态变化。PAbM能够减少组织肥大细胞增多症，这些结果比参考药物（口服200 mg/kg的甘珀酸）所获得的结果更有前景。PAbM的给药能够降低丙二醛水平并提高谷胱甘肽水平，证明了其体内抗氧化活性。因此，本研究报道了PAbM的胃保护潜力，并鼓励在临床前背景下进行进一步研究，以更好地理解与该活性相关的机制及其在旨在治疗胃溃疡的新疗法的临床试验中的应用。
