Pires Maria Luiza Barucci Araujo, Mendes Soares Igor Paulino, Fernandes Lídia de Oliveira, Anselmi Caroline, Gibin Mariana Sversut, Baesso Mauro Luciano, Scheffel Débora Lopes Salles, de Souza Costa Carlos Alberto, Hebling Josimeri
Department of Morphology, Orthodontics, and Pediatric Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil.
Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil.
J Dent. 2025 Oct;161:105993. doi: 10.1016/j.jdent.2025.105993. Epub 2025 Jul 21.
To evaluate the remineralizing potential of biomimetic analogs applied to caries-affected dentin (CAD) using the simulated body fluid (SBF) and a remineralization solution as Ca and PO ions sources.
Flat dentin surfaces from caries-free molars (N = 111) were subjected to a biological protocol (Streptococcus mutans) to induce dentin caries in vitro. The mineral and organic content of sound (SD), infected (ID), CAD, and CAD etched with phosphoric acid (CAD-PA) were determined to validate the protocol. After that, CAD-PA was treated with 100,000 µg/mL sodium trimetaphosphate (STMP) and 500 µg/mL polyacrylic acid (PAA), alone or combined (n = 6/group), applied for 1 min. Teeth were kept under intrapulpal pressure with SBF for 14 days. Next, the mineral-to-matrix ratio (MMR) and surface morphology were evaluated by ATR-FTIR and SEM. Statistical analyses were performed using RM-ANOVA/Sidak and Kruskal-Wallis (α = 5%).
The MMR was statistically different among the dentin substrates (p < 0.001). CAD showed a lower MMR than SD; the lowest MMR was seen when CAD was etched with PA (CAD-PA). However, no change in MMR was observed 14 days after the application of STMP, PAA, or STMP+PAA on CAD-PA.
The microbiological protocol produced a carious dentin that could be partially removed, resulting in a CAD-like substrate. Also, the application of the biomimetic analogs was ineffective in increasing the surface mineral content of the etched CAD after 14 days, even when a REM solution was used as a source of calcium and phosphate ions.
The functional remineralization of caries-affected dentin using the dentin fluid and a mineralizing solution as sources of calcium and phosphate ions did not improve using biomimetic analogs of non-collagenous dentin proteins.
以模拟体液（SBF）和作为钙及磷酸根离子来源的再矿化溶液，评估应用于龋损牙本质（CAD）的仿生类似物的再矿化潜力。
从无龋磨牙（N = 111）获取的平坦牙本质表面，按照生物学方案（变形链球菌）在体外诱导牙本质龋。测定健康牙本质（SD）、感染牙本质（ID）、CAD以及用磷酸蚀刻的CAD（CAD-PA）的矿物质和有机成分，以验证该方案。之后，将CAD-PA分别用100,000 µg/mL的三聚偏磷酸钠（STMP）和500 µg/mL的聚丙烯酸（PAA）单独或联合处理（每组n = 6），处理1分钟。牙齿在牙髓腔内压力下于SBF中保存14天。接下来，通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和扫描电子显微镜（SEM）评估矿物质与基质的比例（MMR）及表面形态。采用重复测量方差分析/西德克检验和克鲁斯卡尔-沃利斯检验进行统计分析（α = 5%）。
牙本质底物之间的MMR存在统计学差异（p < 0.001）。CAD的MMR低于SD；在用PA蚀刻CAD时（CAD-PA）观察到最低的MMR。然而，在CAD-PA上应用STMP、PAA或STMP + PAA 14天后，未观察到MMR有变化。
微生物学方案产生了一种可部分去除的龋坏牙本质，形成类似CAD的底物。此外，即使使用再矿化溶液作为钙和磷酸根离子的来源，仿生类似物在应用14天后对增加蚀刻后CAD的表面矿物质含量也无效。
以牙本质液和矿化溶液作为钙和磷酸根离子来源，使用非胶原蛋白牙本质蛋白的仿生类似物并不能改善龋损牙本质的功能性再矿化。
