Bai Ruijie, Wang Tao, Gu Rongrong, Cai Yawei, Chen Juntao, Cai Wen, Zhou Dianshuang, Li Ying, Luo Jixun, Wang Xiangming, Zhu Zuobin
Jiangsu Engineering Center for Precision Diagnosis and Treatment Research of Polygenic Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical University, Xuzhou, China.
The First Clinical College, Xuzhou Medical University, Xuzhou, China.
Gut Microbes. 2025 Dec;17(1):2536091. doi: 10.1080/19490976.2025.2536091. Epub 2025 Jul 23.
The co-evolutionary relationship between gut microbiota and their hosts is influenced by microbial genetic variation, which enables adaptation to host environmental changes, modifies metabolic processes, and refines host-microbiota interactions. Investigating how gut microbial genetic variations influence host neurobehavior can provide insights into the pathogenesis of neurological disorders. In this study, we screened a comprehensive single-gene knockout library of () and identified 370 mutant strains that reduced social behavior in (). Notably, five mutations in the L-tyrosine biosynthesis pathway significantly alter the social aggregation behavior of via the TGF-β signaling pathway. These findings highlight the importance of considering both microbial genetic variation and community composition in the examination of gut microbe-host neurobehavioral interactions. The establishment of this relationship provides a reference and experimental basis for the development of genetically engineered probiotics aimed at regulating host behavior.
肠道微生物群与其宿主之间的共同进化关系受微生物遗传变异的影响，这种变异能够使微生物适应宿主环境变化、改变代谢过程并优化宿主与微生物群之间的相互作用。研究肠道微生物遗传变异如何影响宿主神经行为能够为神经疾病的发病机制提供见解。在本研究中，我们筛选了一个全面的单基因敲除文库（），并鉴定出370个在（）中会降低社交行为的突变菌株。值得注意的是，L-酪氨酸生物合成途径中的五个突变通过TGF-β信号通路显著改变了（）的社会聚集行为。这些发现凸显了在研究肠道微生物与宿主神经行为相互作用时考虑微生物遗传变异和群落组成的重要性。这种关系的建立为开发旨在调节宿主行为的基因工程益生菌提供了参考和实验依据。
