Wu Xuewan, Liu Yanting, Xiao Ziyi, Deng Ruyi, Huang Rui, Luo Hongjun, Yuan Kaisong
Bio-Analytical Laboratory, Shantou University Medical College Shantou 515041 China
RSC Adv. 2025 Jul 23;15(32):26403-26411. doi: 10.1039/d5ra03108g. eCollection 2025 Jul 21.
Herein, we developed a three-dimensional (3D)-printing fluorescence detector that could be connected to the HPLC system, and distinguished by its ease of assembly, cost-effectiveness, and high adaptability. The detector comprises a laser diode, a 3D-printing flow cell, quartz glass, a long-pass filter, a silicon phototube, and a voltmeter. Technically, the flow cell we designed here has a very compact structure, and it is convenient to embed the optical filter and the phototube, which is also a highlight of our detector to ensure the miniaturization of the whole device. Through the optimization of the detector's structural configuration, the adjustment of the various dimensions and relative positions of the detection window, and the removal of the metal encapsulation cover of the silicon phototransistor, we achieved the optimal detection sensitivity and stability. Experimental findings indicate that the 3D-printing fluorescence detector achieves a limit of detection (LOD) of 5 μg mL, a linear detection range of 5-60 μg mL, and exhibits reasonable inter-day and intra-day stability. Finally, the detector demonstrated its practical utility by successfully separating and detecting a mixed solution of rhodamine 6G and rhodamine B, underscoring its potential for real-world applications.
在此，我们开发了一种可连接到高效液相色谱（HPLC）系统的三维（3D）打印荧光检测器，其特点是易于组装、成本效益高且适应性强。该检测器包括一个激光二极管、一个3D打印流通池、石英玻璃、一个长波通滤光片、一个硅光电管和一个电压表。从技术上讲，我们在此设计的流通池结构非常紧凑，便于嵌入光学滤光片和光电管，这也是我们检测器的一个亮点，可确保整个设备的小型化。通过优化检测器的结构配置、调整检测窗口的各种尺寸和相对位置以及去除硅光电晶体管的金属封装盖，我们实现了最佳的检测灵敏度和稳定性。实验结果表明，3D打印荧光检测器的检测限（LOD）为5 μg/mL，线性检测范围为5 - 60 μg/mL，并且具有合理的日间和日内稳定性。最后，该检测器通过成功分离和检测罗丹明6G和罗丹明B的混合溶液展示了其实际应用价值，突出了其在实际应用中的潜力。
