Hong Sooji, Jung Bong-Kwang, Yang Hyun-Jong
Department of Parasitology, Ewha Womans University School of Medicine, Seoul, Korea.
Ewha Medical Research Center, Ewha Womans University School of Medicine, Seoul, Korea.
Ewha Med J. 2025 Apr;48(2):e29. doi: 10.12771/emj.2025.00311. Epub 2025 Apr 8.
This study aimed to investigate whether proteins present in the molting membranes of third-stage (L3) larvae could serve as potential risk factors for allergic reactions.
Third-stage larvae (L3) of spp. were primarily collected from mackerels and cultured in vitro to yield both molting membranes and fourth-stage (L4) larvae. Major soluble proteins in the molting membranes were identified using SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Crude antigens extracted from L3, L4, and the molting membranes were subsequently evaluated by western blotting using sera from -infected rabbits and patients with eosinophilia.
Antigens derived from the molting membranes reacted with sera from -infected rabbits as well as with sera from 7 patients with eosinophilia of unknown origin. These findings suggest that unidentified proteins in the molting membranes of L3 may contribute to early allergic reactions, particularly in patients sensitized by specific molecular components.
Our results indicate that proteins present in the molting membranes of third-stage spp. larvae may be associated with allergic responses. Further studies are required to confirm the correlation between these membranes and -induced allergies.
本研究旨在调查三期（L3）幼虫蜕皮膜中的蛋白质是否可能作为过敏反应的潜在危险因素。
主要从鲭鱼中收集 spp. 的三期幼虫（L3），并在体外培养以获得蜕皮膜和四期（L4）幼虫。使用 SDS - 聚丙烯酰胺凝胶电泳（SDS - PAGE）鉴定蜕皮膜中的主要可溶性蛋白质。随后，使用来自感染 的兔子和嗜酸性粒细胞增多症患者的血清，通过蛋白质印迹法评估从 L3、L4 和蜕皮膜中提取的粗抗原。
来自蜕皮膜的抗原与感染 的兔子的血清以及 7 名不明原因嗜酸性粒细胞增多症患者的血清发生反应。这些发现表明，L3 蜕皮膜中未鉴定的蛋白质可能导致早期过敏反应，特别是在被特定分子成分致敏的患者中。
我们的结果表明，三期 spp. 幼虫蜕皮膜中的蛋白质可能与过敏反应有关。需要进一步研究以证实这些膜与 诱导的过敏之间的相关性。
