Ghasemzadeh Rahbardar Mahboobeh, Taghavizadeh Yazdi Mohammad Ehsan, Beigoli Sima, Amin Hamideh, Boskabady Mohammad Hossein
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2025;28(7):852-859. doi: 10.22038/ijbms.2025.82057.17753.
We investigated the effects of curcumin, nanocurcumin, and pioglitazone, a peroxisome proliferator-activated receptor gamma (PPARγ) activator, on the systemic inflammation and oxidative stress induced by inhaled paraquat (PQ).
The experimental design included male Wistar rats divided into nine groups. Animals of the control group (Ctrl) were exposed to saline and those of other groups to 54 mg/m PQ aerosols 8 times on alternate days. PQ exposing groups were treated with saline (PQ group), curcumin (30 mg/kg, Cu), nanocurcumin (2 and 8 mg/kg, NC-L, and NC-H), pioglitazone (5 mg/kg, Pio), Pio+ Cu-L, Pio + NC-L, and dexamethasone (0.03 mg/kg, Dexa). Pio was administered intraperitoneally and other treating agents by gavage for 16 days during the PQ exposure period. Total and differential white blood cell (WBC) counts, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), thiol, interleukin (IL)-10, and tumor necrosis factor-alpha (TNF-α) levels were measured.
The inhalation of PQ increased total WBC, differential WBC, MDA, IL-10, and TNF-α blood levels. It also decreased blood levels of CAT, SOD, and thiol. The treatment groups (Cu, NC-L, NC-H, Pio+Cu, Pio+NC-L, Pio, and Dexa) ameliorated PQ-induced alterations. Furthermore, the improvements in most parameters in the Pio+Cu and NC-L-treated group were more significant than the results of the three substances individually.
The amelioration of systemic inflammation and oxidative stress caused by inhaled PQ by Cu, NC, and Pio were shown. Furthermore, the findings indicated a synergistic effect between Pio with Cu and NC, suggesting the involvement of PPARγ-mediated mechanisms in the effects of curcumin.
我们研究了姜黄素、纳米姜黄素以及过氧化物酶体增殖物激活受体γ（PPARγ）激动剂吡格列酮对吸入百草枯（PQ）所致全身炎症和氧化应激的影响。
实验设计包括将雄性Wistar大鼠分为九组。对照组（Ctrl）动物暴露于生理盐水中，其他组动物每隔一天暴露于54 mg/m³的PQ气雾剂中8次。PQ暴露组分别用生理盐水（PQ组）、姜黄素（30 mg/kg，Cu）、纳米姜黄素（2和8 mg/kg，NC-L和NC-H）、吡格列酮（5 mg/kg，Pio）、Pio + Cu-L、Pio + NC-L以及地塞米松（0.03 mg/kg，Dexa）进行处理。在PQ暴露期间，Pio通过腹腔注射给药，其他处理剂通过灌胃给药，持续16天。检测白细胞总数及分类计数、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、巯基、白细胞介素（IL）-10以及肿瘤坏死因子-α（TNF-α）水平。
吸入PQ可使白细胞总数、白细胞分类计数、MDA、IL-10以及TNF-α的血液水平升高。同时还降低了血液中CAT、SOD以及巯基的水平。各治疗组（Cu、NC-L、NC-H、Pio+Cu、Pio+NC-L、Pio以及Dexa）改善了PQ所致的改变。此外，Pio+Cu和NC-L治疗组中大多数参数的改善比这三种物质单独使用时更为显著。
研究表明Cu、NC以及Pio可改善吸入PQ所致的全身炎症和氧化应激。此外，研究结果表明Pio与Cu和NC之间存在协同效应，提示PPARγ介导的机制参与了姜黄素的作用过程。
