Li Chutian, Qiu Sumei, Yang Can, Zhou Runyang, Yuan Yingdan
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China.
J Sci Food Agric. 2025 Jul 24. doi: 10.1002/jsfa.70073.
Dendrobium is an important medicinal plant. There is a significant variation in the medicinal efficacy among different species of Dendrobium. Endophytic microorganisms are crucial in determining the medicinal value of Dendrobium. We investigated the relationship between the endophytic microbial communities and accumulation of active components in Dendrobium by analyzing the accumulation of effective components, the composition of endophytic bacteria and fungi, and their inter-relationships, along with the potential functional predictions in 24 species of Dendrobium and their potential efficacy.
Endophytic microbial communities differ across different Dendrobium species, and the diversity and richness of these communities are correlated with the accumulation of active components in Dendrobium. The diversity of endophytic bacteria correlates negatively with the accumulation of Dendrobium polysaccharides, whereas the richness and diversity of endophytic fungi are positively correlated with the accumulation of Dendrobium alkaloids. Pseudomonas, a dominant species with a high relative abundance across all 24 species of Dendrobium, may exert anti-inflammatory and antibacterial effects. Clostridium sensu stricto, Diaporthe, Cyphellophora and Colletotrichum are correlated with the accumulation of active components in Dendrobium. Endophytic bacteria such as Pseudomonas and Mesorhizobium, and endophytic fungi such as Fusarium and Aspergillus, showed a positive correlation with flavonoid accumulation.
The findings of the present study provide a reference for selecting microorganisms that promote the accumulation of effective substances in Dendrobium and the exploitation of the medicinal resources of Dendrobium. © 2025 Society of Chemical Industry.
石斛是一种重要的药用植物。不同种类的石斛在药用功效上存在显著差异。内生微生物在决定石斛的药用价值方面起着关键作用。我们通过分析24种石斛有效成分的积累、内生细菌和真菌的组成及其相互关系，以及潜在功能预测，研究了内生微生物群落与石斛中活性成分积累之间的关系及其潜在功效。
不同种类的石斛内生微生物群落存在差异，这些群落的多样性和丰富度与石斛中活性成分的积累相关。内生细菌的多样性与石斛多糖的积累呈负相关，而内生真菌的丰富度和多样性与石斛生物碱的积累呈正相关。假单胞菌是所有24种石斛中相对丰度较高的优势种，可能具有抗炎和抗菌作用。严格梭菌属、间座壳属、瓶霉属和炭疽菌属与石斛中活性成分的积累相关。假单胞菌和中生根瘤菌等内生细菌，以及镰刀菌和曲霉等内生真菌，与黄酮类化合物的积累呈正相关。
本研究结果为筛选促进石斛有效物质积累的微生物及石斛药用资源的开发提供了参考。© 2025化学工业协会。
