Zhang Xinyue, Sun Ruijia, Zhan Fan, Yang Guobin, Wang Yi, Yu Yuehua, Ni Zhiyong
Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Organisms, College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, PR China.
College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, PR China.
Plant Sci. 2025 Oct;359:112679. doi: 10.1016/j.plantsci.2025.112679. Epub 2025 Jul 22.
MicroRNAs (miRNAs) are key regulators of F-box-mediated signal transduction in plants and play important roles in the stress response. The ways in which miRNAs target F-box genes in soybeans under salt stress are currently unknown. In this study, the functional role of GmFBX193, the gene target of gma-miR4359b, under salt stress was investigated. The length, surface area ratio, survival rate, proline content, superoxide dismutase (SOD) activity and peroxidase (POD) activity of soybean hairy roots with inhibited GmFBX193 expression were greater than those of the K599 reference root samples. In contrast, the root length, seed germination rate, number of green cotyledons, chlorophyll content, catalase activity, SOD activity and POD activity of transgenic Arabidopsis plants overexpressing GmFBX193 were lower than those of wild-type (WT) Arabidopsis plants. Under salt stress, compared with WT Arabidopsis control plants, GmFBX193 transgenic Arabidopsis plants presented 239 differentially expressed genes, including several known salt stress-related genes. Dual-luciferase assays and cotransformation of soybean hairy roots revealed that gma-miR4359b participates in salt tolerance regulation by cleaving GmFBX193. Yeast two-hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assays revealed that the GmFBX193 protein interacted with two proteins, GmSK12 and GmSK15, which are encoded by soybean SKP1 family genes. In summary, gma-miR4359b can target the gene expression of GmFBX193 and respond to salt stress. The inhibition of GmFBX193 can improve the salt tolerance of plants.
微小RNA（miRNA）是植物中F-box介导的信号转导的关键调节因子，在应激反应中发挥重要作用。目前尚不清楚盐胁迫下miRNA靶向大豆中F-box基因的方式。在本研究中，研究了盐胁迫下gma-miR4359b的基因靶标GmFBX193的功能作用。GmFBX193表达受到抑制的大豆毛状根的长度、表面积比、存活率、脯氨酸含量、超氧化物歧化酶（SOD）活性和过氧化物酶（POD）活性均高于K599参考根样本。相反，过表达GmFBX193的转基因拟南芥植株的根长、种子发芽率、绿色子叶数、叶绿素含量、过氧化氢酶活性、SOD活性和POD活性均低于野生型（WT）拟南芥植株。在盐胁迫下，与WT拟南芥对照植株相比，GmFBX193转基因拟南芥植株呈现出239个差异表达基因，包括几个已知的盐胁迫相关基因。双荧光素酶测定和大豆毛状根的共转化表明，gma-miR4359b通过切割GmFBX193参与耐盐性调节。酵母双杂交、双分子荧光互补和免疫共沉淀测定表明，GmFBX193蛋白与大豆SKP1家族基因编码的两种蛋白GmSK12和GmSK15相互作用。总之，gma-miR4359b可以靶向GmFBX193的基因表达并响应盐胁迫。抑制GmFBX193可以提高植物的耐盐性。
