Wang Xinhui, Gao Ming, Kong Yihan, Yu Qian, Yao Lu, Li Xingguo, Li Wenhui, Liu Wanda, Hou Ruining, Zhang Lihua, Han Deguo
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs / National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Horticulture Branch, Heilongjiang Academy of Agricultural Sciences, Harbin 150069, China.
Int J Mol Sci. 2025 Jun 18;26(12):5833. doi: 10.3390/ijms26125833.
The WRKY transcription factor family is a significant family of plant transcription factors (TFs). Plant growth and development are often influenced by abiotic factors, such as salinity and low temperature. Numerous studies have demonstrated that WRKY TFs primarily influence plant responses to adversity. However, there are few studies on the role of WRKY genes in the stress responses of (L.) Borkh. We cloned the gene from for this research, and its roles in salt stress tolerance were analyzed. Phylogenetic tree analysis revealed that MbWRKY33 and PbWRKY33 have the highest homology. Subcellular localization revealed that MbWRKY33 was located within the nucleus. An analysis of tissue-specific expression showed that had relatively high expression levels in young leaves and roots. Moreover, plants overexpressing exhibited stronger resistance to salt stress compared with the wild type (WT) and the unloaded line empty vector (UL). Under the treatment of 200 mM NaCl, transgenic plants exhibited significantly higher activities of antioxidant enzymes like superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) than the control. In contrast, the WT and the UL lines had elevated levels of malondialdehyde (MDA) and reactive oxygen species (ROS). In addition, elevates transgenic plant resistance to salt stress by regulating the expression levels of , and . Results indicated that in might be linked to high-salinity stress responses, laying a foundation for understanding WRKY TFs' reaction to such stress.
WRKY转录因子家族是植物转录因子中一个重要的家族。植物的生长和发育常常受到非生物因素的影响，如盐度和低温。大量研究表明，WRKY转录因子主要影响植物对逆境的反应。然而，关于WRKY基因在枸杞（L.）Borkh.胁迫反应中的作用的研究较少。我们为此研究从枸杞中克隆了该基因，并分析了其在耐盐胁迫中的作用。系统发育树分析表明，MbWRKY33和PbWRKY33具有最高的同源性。亚细胞定位显示MbWRKY33位于细胞核内。组织特异性表达分析表明，该基因在幼叶和根中具有较高的表达水平。此外，与野生型（WT）和空载载体（UL）相比，过表达该基因的枸杞植株对盐胁迫表现出更强的抗性。在200 mM NaCl处理下，转基因枸杞植株的超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等抗氧化酶活性显著高于对照。相反，WT和UL系的丙二醛（MDA）和活性氧（ROS）水平升高。此外，该基因通过调节相关基因的表达水平提高转基因植物对盐胁迫的抗性。结果表明，枸杞中的该基因可能与高盐胁迫反应有关，为理解WRKY转录因子对这种胁迫的反应奠定了基础。
