A Golgi-targeting fluorescent probe for the detection of hydrazine in biological and environmental systems.

Hydrazine (NH) is regarded as an extremely toxic agent but it is used in large quantities as a chemical raw material in various industries, which poses a severe danger to both the environment and public health. Notably, the Golgi apparatus (GA) of hepatocytes is frequently affected by toxins in cases of hepatotoxicity. Therefore, developing a probe to monitor NH in environmental systems and within the GA of live cells is highly valuable. A Golgi-targeting fluorescent probe, BHMBS, is synthesized to detect NH. The probe BHMBS emits faint green fluorescence at 550 nm, which is remarkably enhanced after adding NH. The probe can detect NH with an ultra-short reaction time, high sensitivity, and excellent selectivity, making it a valuable tool to monitor NH in the real world and image the NH fluctuations in cells and zebrafish.