用于检测活细胞、土壤样品中肼的基于香豆素二醛的荧光探针及其在试纸条中的应用。
Coumarin bialdehyde-based fluorescent probe for the detection of hydrazine in living cells, soil samples and its application in test strips.
作者信息
Shan Lin, Li Xuewei, Yu Zhe, Zheng Xiuli, Ren Haohui, Wu Jiasheng, Lv Chuantao, Wang Pengfei, Liu Weimin
机构信息
Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Dec 15;343:126540. doi: 10.1016/j.saa.2025.126540. Epub 2025 Jun 6.
As an essential fine chemical material, the prevalent application of hydrazine (NH) inevitably endangers environmental safety and biological health. Herein, we propose a coumarin derivative (Cou-N-2CHO) with a unique bialdehyde structure for the detection of hydrazine. Both aldehyde groups of Cou-N-2CHO could be nucleophilically attacked by hydrazine, resulting in a new adduct with significant fluorescence enhancement (22-fold). Possible responsive mechanism has been verified by NMR and ESI-MS analyses. Cou-N-2CHO was sensitive to hydrazine with a limit of detection (LOD) as low as 1.02 μM. Moreover, Cou-N-2CHO showed good specificity and excellent immunity to interference in response to hydrazine. Apart from successfully enabling cell imaging of hydrazine, Cou-N-2CHO can also be applied in tests of paper strips and soil analysis. More meaningfully, this work opens up more possibilities to detect hydrazine in multiple environmental samples.
作为一种重要的精细化工原料，肼（NH）的广泛应用不可避免地危及环境安全和生物健康。在此，我们提出一种具有独特双醛结构的香豆素衍生物（Cou-N-2CHO）用于检测肼。Cou-N-2CHO的两个醛基均可被肼亲核进攻，形成一种具有显著荧光增强（22倍）的新加合物。通过核磁共振（NMR）和电喷雾电离质谱（ESI-MS）分析验证了可能的响应机制。Cou-N-2CHO对肼敏感，检测限（LOD）低至1.02 μM。此外，Cou-N-2CHO对肼表现出良好的特异性和出色的抗干扰能力。除了成功实现肼的细胞成像外，Cou-N-2CHO还可应用于试纸测试和土壤分析。更有意义的是，这项工作为在多种环境样品中检测肼开辟了更多可能性。
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