Shan Lin, Li Xuewei, Yu Zhe, Zheng Xiuli, Ren Haohui, Wu Jiasheng, Lv Chuantao, Wang Pengfei, Liu Weimin
Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Dec 15;343:126540. doi: 10.1016/j.saa.2025.126540. Epub 2025 Jun 6.
As an essential fine chemical material, the prevalent application of hydrazine (NH) inevitably endangers environmental safety and biological health. Herein, we propose a coumarin derivative (Cou-N-2CHO) with a unique bialdehyde structure for the detection of hydrazine. Both aldehyde groups of Cou-N-2CHO could be nucleophilically attacked by hydrazine, resulting in a new adduct with significant fluorescence enhancement (22-fold). Possible responsive mechanism has been verified by NMR and ESI-MS analyses. Cou-N-2CHO was sensitive to hydrazine with a limit of detection (LOD) as low as 1.02 μM. Moreover, Cou-N-2CHO showed good specificity and excellent immunity to interference in response to hydrazine. Apart from successfully enabling cell imaging of hydrazine, Cou-N-2CHO can also be applied in tests of paper strips and soil analysis. More meaningfully, this work opens up more possibilities to detect hydrazine in multiple environmental samples.
作为一种重要的精细化工原料，肼（NH）的广泛应用不可避免地危及环境安全和生物健康。在此，我们提出一种具有独特双醛结构的香豆素衍生物（Cou-N-2CHO）用于检测肼。Cou-N-2CHO的两个醛基均可被肼亲核进攻，形成一种具有显著荧光增强（22倍）的新加合物。通过核磁共振（NMR）和电喷雾电离质谱（ESI-MS）分析验证了可能的响应机制。Cou-N-2CHO对肼敏感，检测限（LOD）低至1.02 μM。此外，Cou-N-2CHO对肼表现出良好的特异性和出色的抗干扰能力。除了成功实现肼的细胞成像外，Cou-N-2CHO还可应用于试纸测试和土壤分析。更有意义的是，这项工作为在多种环境样品中检测肼开辟了更多可能性。
