Xie Yongjun, Qin Wenlian, Wang Mengjia, Pan Xiaozhuo, Qin Xiaojie, Wang Yibing
Guangxi Key Laboratory for Polysaccharide Materials and Modifications, College of Marine and Biotechnology, Guangxi Minzu University, Nanning, China.
Front Microbiol. 2025 Jul 10;16:1627112. doi: 10.3389/fmicb.2025.1627112. eCollection 2025.
As the years of mango cultivation progress, pathogens invade the soil, leading to the development of soil borne diseases. These diseases not only change the physical and chemical properties of the soil but also influence the diversity and composition of soil microbes, ultimately impeding the development of the mango industry. In view of this, this study aimed to explore the correlations among the physical and chemical properties of mango root soil, root exudates, soil microbial community and soil borne diseases.
Healthy, diseased and severely diseased mango root soil samples were taken as the investigation objects. The main research methods were: (1)Testing seven soil physicochemical properties, such as total phosphorus and total potassium, in rhizosphere soil. (2) We determined the phenolic acid content in mango rhizosphere soil using high-performance liquid chromatography (HPLC). (3) Soil microbial communities were analyzed using second-generation high-throughput sequencing technology. (4) The characteristics and response mechanisms of changes in soil microbial community structure were analyzed using multivariate statistical methods, such as redundancy analysis (RDA) and correlation analysis, in combination with physical and chemical environmental factors. (5) PICRUSt2 analysis of microbial community function under soil borne diseases.
Soil borne disease had profound impacts on soil physicochemical properties, root exudates (phenolic acid) and microbial community structure. On one hand, with the development of soil-borne disease, the mango's ability to absorb foreign nutrients is weakened, leading to the accumulation of nutrients in the root soil, which significantly increases total phosphorus, total potassium, alkaline hydrolysis nitrogen, acid-soluble phosphorus, available potassium, organic matter and pH value. On the other hand, soil borne disease also increased the secretion of phenolic acid in mango root, with significantly increased concentrations of vanillic acid, ferulic acid, salicylic acid and coumaric acid. High-throughput sequencing results showed that soil-borne diseases were followed by a decrease in bacterial diversity, an increase in fungal diversity, and the accumulation of pathogenic microorganisms such as Fusarium in the soil. In addition, the physical and chemical properties of the soil as well as phenolic acid exudates also influenced microbial community functioning, particularly with respect to genetic information processing, metabolism and biological systems.
In this study, we investigated the mechanism of soil-borne diseases in mango by studying the response mechanism of soil-borne diseases with root secretion and microbial community. It provides theoretical support for the sustainable development of mango industry.
随着芒果种植年限的增加，病原菌侵入土壤，导致土传病害的发生。这些病害不仅改变了土壤的理化性质，还影响了土壤微生物的多样性和组成，最终阻碍了芒果产业的发展。鉴于此，本研究旨在探讨芒果根际土壤理化性质、根系分泌物、土壤微生物群落与土传病害之间的相关性。
以健康、患病和重病芒果根际土壤样本为研究对象。主要研究方法包括：（1）测定根际土壤中全磷、全钾等七种土壤理化性质。（2）采用高效液相色谱法（HPLC）测定芒果根际土壤中酚酸含量。（3）利用第二代高通量测序技术分析土壤微生物群落。（4）结合理化环境因素，采用冗余分析（RDA）和相关性分析等多元统计方法分析土壤微生物群落结构变化的特征及响应机制。（5）对土传病害下微生物群落功能进行PICRUSt2分析。
土传病害对土壤理化性质、根系分泌物（酚酸）和微生物群落结构产生了深远影响。一方面，随着土传病害的发展，芒果吸收外源养分的能力减弱，导致根际土壤中养分积累，全磷、全钾、碱解氮、酸溶性磷、速效钾、有机质和pH值显著增加。另一方面，土传病害还增加了芒果根中酚酸的分泌，香草酸、阿魏酸、水杨酸和香豆酸的浓度显著增加。高通量测序结果表明，土传病害导致细菌多样性降低，真菌多样性增加，土壤中镰刀菌等致病微生物积累。此外，土壤理化性质以及酚酸分泌物也影响微生物群落功能，特别是在遗传信息处理、代谢和生物系统方面。
本研究通过研究土传病害与根系分泌物和微生物群落的响应机制，探讨芒果土传病害的发生机理。为芒果产业的可持续发展提供了理论支持。
